Literature DB >> 2747621

Isolation and characterization of an ipt gene from the Ti plasmid Bo542.

T J Strabala1, S Y Bednarek, G Bertoni, R M Amasino.   

Abstract

A 1.9 kb clone of the T-DNA region of the Agrobacterium tumefaciens Ti plasmid Bo542 which exhibited homology to the isopentenyl transferase (ipt) locus of pTiA6 was identified by low stringency DNA hybridization. Introduction of this segment of pTiBo542 DNA into cells of Nicotiana tabacum or N. glauca caused tumor formation in vivo, and allowed hormone independent growth in vitro. Furthermore, this DNA segment complemented ipt mutant strains of A. tumefaciens, restoring their ability to cause tumors on Kalanchöe leaves and tomato stems. The complete DNA sequence of this segment has been determined, revealing an open reading frame homologous to other known Agrobacterium ipt genes.

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Year:  1989        PMID： 2747621     DOI： 10.1007/bf00334380

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  21 in total

1.  Virulence of Agrobacterium tumefaciens Strain A281 on Legumes.

Authors:  E E Hood; R T Fraley; M D Chilton
Journal:  Plant Physiol       Date:  1987-03       Impact factor: 8.340

2.  Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955.

Authors:  R F Barker; K B Idler; D V Thompson; J D Kemp
Journal:  Plant Mol Biol       Date:  1983-11       Impact factor: 4.076

3.  Expression of an Agrobacterium Ti plasmid gene involved in cytokinin biosynthesis is regulated by virulence loci and induced by plant phenolic compounds.

Authors:  M C John; R M Amasino
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

4.  Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors:  E Y Chen; P H Seeburg
Journal:  DNA       Date:  1985-04

5.  T-DNA and opine synthetic loci in tumors incited by Agrobacterium tumefaciens A281 on soybean and alfalfa plants.

Authors:  E E Hood; W S Chilton; M D Chilton; R T Fraley
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

6.  Hormonal control of tobacco crown gall tumor morphology.

Authors:  R M Amasino; C O Miller
Journal:  Plant Physiol       Date:  1982-02       Impact factor: 8.340

7.  Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.

Authors:  D J Garfinkel; E W Nester
Journal:  J Bacteriol       Date:  1980-11       Impact factor: 3.490

8.  Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti.

Authors:  S A Leong; G S Ditta; D R Helinski
Journal:  J Biol Chem       Date:  1982-08-10       Impact factor: 5.157

9.  Deoxyribonuclease I sensitivity of the T-DNA ipt gene is associated with gene expression.

Authors:  R A Reid; M C John; R M Amasino
Journal:  Biochemistry       Date:  1988-07-26       Impact factor: 3.162

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  7 in total

1.  Levels and location of expression of the Agrobacterium tumefaciens pTiA6 ipt gene promoter in transgenic tobacco.

Authors:  T J Strabala; D N Crowell; R M Amasino
Journal:  Plant Mol Biol       Date:  1993-03       Impact factor: 4.076

2.  In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco.

Authors:  S T Neuteboom; E Hulleman; R A Schilperoort; J H Hoge
Journal:  Plant Mol Biol       Date:  1993-08       Impact factor: 4.076

3.  Mapping and genetic organization of pTiChry5, a novel Ti plasmid from a highly virulent Agrobacterium tumefaciens strain.

Authors:  L G Kovács; S G Pueppke
Journal:  Mol Gen Genet       Date:  1994-02

4.  Agrobacterium vitis nopaline Ti plasmid pTiAB4: relationship to other Ti plasmids and T-DNA structure.

Authors:  L Otten; P De Ruffray
Journal:  Mol Gen Genet       Date:  1994-11-15

5.  Genetic characterization of a double-flowered tobacco plant obtained in a transformation experiment.

Authors:  T Komari
Journal:  Theor Appl Genet       Date:  1990-08       Impact factor: 5.699

Review 6.  It's Hard to Avoid Avoidance: Uncoupling the Evolutionary Connection between Plant Growth, Productivity and Stress "Tolerance".

Authors:  Albino Maggio; Ray A Bressan; Yang Zhao; Junghoon Park; Dae-Jin Yun
Journal:  Int J Mol Sci       Date:  2018-11-20       Impact factor: 5.923

7.  Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.

Authors:  Sundaram Kuppu; Neelam Mishra; Rongbin Hu; Li Sun; Xunlu Zhu; Guoxin Shen; Eduardo Blumwald; Paxton Payton; Hong Zhang
Journal:  PLoS One       Date:  2013-05-10       Impact factor: 3.240
  7 in total

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