| Literature DB >> 27464797 |
Colleen A Brady1,2,3, Andrew J Rennekamp1,2,3, Randall T Peterson4,5,6.
Abstract
Phenotypic small molecule screens in zebrafish have gained popularity as an unbiased approach to probe biological processes. In this chapter we outline basic methods for performing chemical screens with larval zebrafish including breeding large numbers of embryos, plating larval fish into multi-well dishes, and adding small molecules to these wells. We also highlight important considerations when designing and interpreting the results of a phenotypic screen and possible follow-up approaches, including popular methods used to identify the mechanism of action of a chemical compound.Entities:
Keywords: Multi-well plates; Phenotypic screening; Small molecules
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Year: 2016 PMID： 27464797 DOI： 10.1007/978-1-4939-3771-4_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745