| Literature DB >> 2746161 |
J S Economou1, K Rhoades, R Essner, W H McBride, J C Gasson, D L Morton.
Abstract
The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient transfection system using the TNF-alpha-producing U937 cell line. Full-length and truncated TNF promoter constructions extending from -615 to -95 bp relative to the transcription start site (TSS) could be induced by phorbol esters. A construct truncated to within 36 bp of the TSS (and within 11 bp of the TATAA box) was inactive. Therefore, the phorbol ester responsive is localized in the TNF/cachectin promoter to a relatively short region proximal to the TATAA box.Entities:
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Year: 1989 PMID： 2746161 PMCID： PMC2189360 DOI： 10.1084/jem.170.1.321
Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307