Isabel Fort-Gallifa1, Anabel García-Heredia2, Anna Hernández-Aguilera2, Josep M Simó3, Julio Sepúlveda4, Vicente Martín-Paredero4, Jordi Camps5, Jorge Joven2. 1. Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; Laboratori de Referència de Catalunya SUD, Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain. 2. Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain. 3. Laboratori de Referència de Catalunya SUD, Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain. 4. Servei d'Angiologia, Cirurgia Vascular i Endocirurgia, Hospital Universitari Joan XXIII, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Tarragona, Spain. 5. Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d'Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Spain. Electronic address: jcamps@grupsagessa.com.
Abstract
BACKGROUND: The aims of this study were: (1) to investigate changes in indices of oxidative stress and inflammation in the evaluation of peripheral artery disease (PAD); (2) to compare the diagnostic efficacy of these parameters with that of classical clinical laboratory routine parameters. DESIGN AND METHODS: We studied 115 patients with PAD and 300 healthy volunteers. RESULTS: PAD patients had significantly increased circulating concentrations of F2-isoprostanes, protein carbonyls, chemokine (C-C motif) ligand 2 (CCL2), high-sensitivity C-reactive protein (hs-CRP), β-2-microglobulin (B2M), and decreased paraoxonase-1 (PON1) levels. When patients were classified according to the Fontaine score, we observed important increases in plasma F2-isoprostanes and CCL2 that appeared in milder stages of the disease, and remained so at similar levels in more advanced stages; almost no overlapping with the control group was noted. Receiver operating characteristics analysis comparing patients and controls revealed that the areas under the curve for F2-isoprostanes and CCL2 approached unity [0.999 (0.998-1.000) and 0.993 (0.985-1.000)], respectively, and significantly higher to those of the other measured parameters. CONCLUSION: Our data suggest that F2-isoprostanes and CCL2 measurements may be useful tools for the diagnosis of PAD.
BACKGROUND: The aims of this study were: (1) to investigate changes in indices of oxidative stress and inflammation in the evaluation of peripheral artery disease (PAD); (2) to compare the diagnostic efficacy of these parameters with that of classical clinical laboratory routine parameters. DESIGN AND METHODS: We studied 115 patients with PAD and 300 healthy volunteers. RESULTS: PAD patients had significantly increased circulating concentrations of F2-isoprostanes, protein carbonyls, chemokine (C-C motif) ligand 2 (CCL2), high-sensitivity C-reactive protein (hs-CRP), β-2-microglobulin (B2M), and decreased paraoxonase-1 (PON1) levels. When patients were classified according to the Fontaine score, we observed important increases in plasma F2-isoprostanes and CCL2 that appeared in milder stages of the disease, and remained so at similar levels in more advanced stages; almost no overlapping with the control group was noted. Receiver operating characteristics analysis comparing patients and controls revealed that the areas under the curve for F2-isoprostanes and CCL2 approached unity [0.999 (0.998-1.000) and 0.993 (0.985-1.000)], respectively, and significantly higher to those of the other measured parameters. CONCLUSION: Our data suggest that F2-isoprostanes and CCL2 measurements may be useful tools for the diagnosis of PAD.
Authors: Anna Hernández-Aguilera; Núria Casacuberta; Helena Castañé; Montserrat Fibla; Salvador Fernández-Arroyo; Isabel Fort-Gallifa; Marta París; Fàtima Sabench; Daniel Del Castillo; Gerard Baiges-Gaya; Elisabet Rodríguez-Tomàs; Teresa Sans; Jordi Camps; Jorge Joven Journal: Biol Trace Elem Res Date: 2021-02-08 Impact factor: 3.738
Authors: Karl Kuusik; Teele Kasepalu; Mihkel Zilmer; Jaan Eha; Mare Vähi; Liisi Anette Torop; Jüri Lieberg; Jaak Kals Journal: Oxid Med Cell Longev Date: 2021-12-08 Impact factor: 6.543
Authors: Helena Castañé; Simona Iftimie; Gerard Baiges-Gaya; Elisabet Rodríguez-Tomàs; Andrea Jiménez-Franco; Ana Felisa López-Azcona; Pedro Garrido; Antoni Castro; Jordi Camps; Jorge Joven Journal: Metabolism Date: 2022-04-02 Impact factor: 13.934