Nawaporn Vinayavekhin1, Jetjamnong Sueajai2, Nichaboon Chaihad3, Ratchanee Panrak4, Ratchanaporn Chokchaisiri5, Polkit Sangvanich6, Apichart Suksamrarn7, Pawinee Piyachaturawat8. 1. Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Omics Sciences and Bioinformatics Center, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: nawaporn.v@chula.ac.th. 2. Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Electronic address: jetjamnong.sue@mahidol.ac.th. 3. Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: kewyo_za@hotmail.com. 4. Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: yu_iinano@hotmail.com. 5. Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand. Electronic address: pam_2022@hotmail.com. 6. Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: polkit@gmail.com. 7. Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand. Electronic address: s_apichart@ru.ac.th. 8. Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Chakri Naruebodindra Medical Institute, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: pawinee.pia@mahidol.ac.th.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Curcuma comosa Roxb. (C. comosa) or Wan Chak Motluk, Zingiberaceae family, has been used in Thai traditional medicine for the treatment of gynecological problems and inflammation. AIM OF THE STUDY: This study aimed to investigate the therapeutic potential of C. comosa by determining the changes in the lipid profiles in the ovariectomized rats, as a model of estrogen-deficiency-induced hyperlipidemia, after treatment with different components of C. comosa using an untargeted lipidomics approach. MATERIALS AND METHODS: Lipids were extracted from the serum of adult female rats subjected to a sham operation (SHAM; control), ovariectomy (OVX), or OVX with 12-week daily doses of estrogen (17β-estradiol; E2), (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (DPHD; a phytoestrogen from C. comosa), powdered C. comosa rhizomes or its crude ethanol extract. They were then analyzed by liquid chromatography-mass spectrometry, characterized, and subjected to the orthogonal projections to latent structures discriminant analysis statistical model to identify tentative biomarkers. RESULTS: Levels of five classes of lipids (ceramide, ceramide-1-phosphate, sphingomyelin, 1-O-alkenyl-lysophosphatidylethanolamine and lysophosphatidylethanolamine) were elevated in the OVX rats compared to those in the SHAM rats, while the monoacylglycerols and triacylglycerols were decreased. The E2 treatment only reversed the levels of ceramides, whereas treatments with DPHD, C. comosa extract or powder returned the levels of all upregulated lipids back to those in the SHAM control rats. CONCLUSIONS: The findings suggest the potential beneficial effects of C. comosa on preventing the increased ceramide levels in OVX rats, a possible cause of metabolic disturbance under estrogen deficiency. Overall, the results demonstrated the power of untargeted lipidomics in discovering disease-relevant biomarkers, as well as evaluating the effectiveness of treatment by C. comosa components (DPHD, extract or powder) as utilized in Thai traditional medicine, and also providing scientific support for its folklore use.
ETHNOPHARMACOLOGICAL RELEVANCE: Curcuma comosa Roxb. (C. comosa) or Wan Chak Motluk, Zingiberaceae family, has been used in Thai traditional medicine for the treatment of gynecological problems and inflammation. AIM OF THE STUDY: This study aimed to investigate the therapeutic potential of C. comosa by determining the changes in the lipid profiles in the ovariectomized rats, as a model of estrogen-deficiency-induced hyperlipidemia, after treatment with different components of C. comosa using an untargeted lipidomics approach. MATERIALS AND METHODS:Lipids were extracted from the serum of adult female rats subjected to a sham operation (SHAM; control), ovariectomy (OVX), or OVX with 12-week daily doses of estrogen (17β-estradiol; E2), (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (DPHD; a phytoestrogen from C. comosa), powdered C. comosa rhizomes or its crude ethanol extract. They were then analyzed by liquid chromatography-mass spectrometry, characterized, and subjected to the orthogonal projections to latent structures discriminant analysis statistical model to identify tentative biomarkers. RESULTS: Levels of five classes of lipids (ceramide, ceramide-1-phosphate, sphingomyelin, 1-O-alkenyl-lysophosphatidylethanolamine and lysophosphatidylethanolamine) were elevated in the OVX rats compared to those in the SHAM rats, while the monoacylglycerols and triacylglycerols were decreased. The E2 treatment only reversed the levels of ceramides, whereas treatments with DPHD, C. comosa extract or powder returned the levels of all upregulated lipids back to those in the SHAM control rats. CONCLUSIONS: The findings suggest the potential beneficial effects of C. comosa on preventing the increased ceramide levels in OVX rats, a possible cause of metabolic disturbance under estrogen deficiency. Overall, the results demonstrated the power of untargeted lipidomics in discovering disease-relevant biomarkers, as well as evaluating the effectiveness of treatment by C. comosa components (DPHD, extract or powder) as utilized in Thai traditional medicine, and also providing scientific support for its folklore use.
Authors: Diana Cabrera; Marlena Kruger; Frances M Wolber; Nicole C Roy; John J Totman; Christiani Jeyakumar Henry; David Cameron-Smith; Karl Fraser Journal: Int J Environ Res Public Health Date: 2018-05-22 Impact factor: 3.390
Authors: Ismael González-García; Cristina Contreras; Ánxela Estévez-Salguero; Francisco Ruíz-Pino; Benoit Colsh; Iván Pensado; Laura Liñares-Pose; Eva Rial-Pensado; Pablo B Martínez de Morentin; Johan Fernø; Carlos Diéguez; Rubén Nogueiras; Hervé Le Stunff; Christophe Magnan; Manuel Tena-Sempere; Miguel López Journal: Cell Rep Date: 2018-10-09 Impact factor: 9.423
Authors: Riikka Johanna Niemi; Elena Ioana Braicu; Hagen Kulbe; Kaisa Maria Koistinen; Jalid Sehouli; Ulla Puistola; Johanna Unelma Mäenpää; Mika Hilvo Journal: Br J Cancer Date: 2018-10-08 Impact factor: 7.640