| Literature DB >> 27431992 |
Ilya G Kichigin1, Massimo Giovannotti2, Alex I Makunin3, Bee L Ng4, Marsel R Kabilov5, Alexey E Tupikin5, Vincenzo Caputo Barucchi2,6, Andrea Splendiani2, Paolo Ruggeri2, Willem Rens7, Patricia C M O'Brien7, Malcolm A Ferguson-Smith7, Alexander S Graphodatsky3,8, Vladimir A Trifonov3,8.
Abstract
Squamate reptiles show a striking diversity in modes of sex determination, including both genetic (XY or ZW) and temperature-dependent sex determination systems. The genomes of only a handful of species have been sequenced, analyzed and assembled including the genome of Anolis carolinensis. Despite a high genome coverage, only macrochromosomes of A. carolinensis were assembled whereas the content of most microchromosomes remained unclear. Most of the Anolis species have homomorphic XY sex chromosome system. However, some species have large heteromorphic XY chromosomes (e.g., A. sagrei) and even multiple sex chromosomes systems (e.g. A. pogus), that were shown to be derived from fusions of the ancestral XY with microautosomes. We applied next generation sequencing of flow sorting-derived chromosome-specific DNA pools to characterize the content and composition of microchromosomes in A. carolinensis and A. sagrei. Comparative analysis of sequenced chromosome-specific DNA pools revealed that the A. sagrei XY sex chromosomes contain regions homologous to several microautosomes of A. carolinensis. We suggest that the sex chromosomes of A. sagrei are derived by fusions of the ancestral sex chromosome with three microautosomes and subsequent loss of some genetic content on the Y chromosome.Entities:
Keywords: Reptilia; Sex determining (SD) genes; Squamata; XY sex chromosomes
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Year: 2016 PMID： 27431992 DOI： 10.1007/s00438-016-1230-z
Source DB: PubMed Journal: Mol Genet Genomics ISSN： 1617-4623 Impact factor: 3.291