| Literature DB >> 27409454 |
Marius M Haugland1, Afaf H El-Sagheer1,2, Rachel J Porter1, Javier Peña3, Tom Brown1, Edward A Anderson1, Janet E Lovett4.
Abstract
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.Entities:
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Year: 2016 PMID： 27409454 DOI： 10.1021/jacs.6b05421
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419