Literature DB >> 27407304

Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples.

Antonio Meza-Lucas1, María-Fernanda Pérez-Villagómez2, José-Patricio Martínez-López2, Ricardo García-Rodea1, María-Guadalupe Martínez-Castelán2, Alejandro Escobar-Gutiérrez1, Jorge-Luis de-la-Rosa-Arana1, Altagracia Villanueva-Zamudio2.   

Abstract

A comparison of DOT-ELISA and Standard-ELISA was made for detection of Vibrio cholerae toxin in culture supernatants of bacteria isolated from human and environmental samples. A total of 293 supernatants were tested in a double blind assay. A correlation of 100 % was obtained between both techniques. The cholera toxin was found in 20 Inaba and 3 Ogawa strains. Positive samples were from seafood (17 samples), potable water (1 sample) and sewage (5 samples). The DOT-ELISA was useful as the standard-ELISA to confirm the presence of cholera toxin in the environmental samples.

Entities:  

Keywords:  Cholera; DOT-ELISA; Mexico

Year:  2016        PMID： 27407304      PMCID： PMC4920772          DOI： 10.1007/s12088-016-0596-2

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  13 in total

1.  Genome Wide Analysis for Rapid Identification of Vibrio Species.

Authors:  Vipin Chandra Kalia; Prasun Kumar; Ravi Kumar; Anjali Mishra; Shikha Koul
Journal:  Indian J Microbiol       Date:  2015-09-07       Impact factor: 2.461

2.  Occurrence in Mexico, 1998-2008, of Vibrio cholerae CTX+ El Tor carrying an additional truncated CTX prophage.

Authors:  Munirul Alam; Shah Manzur Rashed; Shahnewaj Bin Mannan; Tarequl Islam; Marcial Leonardo Lizarraga-Partida; Gabriela Delgado; Rosario Morales-Espinosa; Jose Luis Mendez; Armando Navarro; Haruo Watanabe; Makoto Ohnishi; Nur A Hasan; Anwar Huq; R Bradley Sack; Rita R Colwell; Alejandro Cravioto
Journal:  Proc Natl Acad Sci U S A       Date:  2014-06-23       Impact factor: 11.205

3.  Epidemic risk from cholera introductions into Mexico.

Authors:  Sean M Moore; Kerry L Shannon; Carla E Zelaya; Andrew S Azman; Justin Lessler
Journal:  PLoS Curr       Date:  2014-02-21

4.  Screening of aquatic samples for Vibrio cholerae serotype O1 by a dot-blot method and a latex agglutination test.

Authors:  Y Nishikawa; A Hase; E Ishii; T Kishi
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

5.  Identification and strain differentiation of Vibrio cholerae by using polyclonal antibodies against outer membrane proteins.

Authors:  A Martínez-Govea; J Ambrosio; L Gutiérrez-Cogco; A Flisser
Journal:  Clin Diagn Lab Immunol       Date:  2001-07

6.  Quantitative detection of Vibrio cholera toxin by real-time and dynamic cytotoxicity monitoring.

Authors:  Dazhi Jin; Yun Luo; Min Zheng; Haijing Li; Jing Zhang; Melinda Stampfl; Xiao Xu; Gangqiang Ding; Yanjun Zhang; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948

7.  Rapid diagnosis of cholera caused by Vibrio cholerae O139.

Authors:  W Chaicumpa; P Srimanote; Y Sakolvaree; T Kalampaheti; M Chongsa-Nguan; P Tapchaisri; B Eampokalap; P Moolasart; G B Nair; P Echeverria
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948

8.  Rapid simultaneous ultrasensitive immunodetection of five bacterial toxins.

Authors:  Yuri M Shlyapnikov; Elena A Shlyapnikova; Maria A Simonova; Anna O Shepelyakovskaya; Fedor A Brovko; Ravilya L Komaleva; Eugene V Grishin; Victor N Morozov
Journal:  Anal Chem       Date:  2012-06-18       Impact factor: 6.986

9.  Economic burden of cholera in the WHO African region.

Authors:  Joses M Kirigia; Luis G Sambo; Allarangar Yokouide; Edoh Soumbey-Alley; Lenity K Muthuri; Doris G Kirigia
Journal:  BMC Int Health Hum Rights       Date:  2009-04-30

10.  Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification.

Authors:  Wataru Yamazaki; Kazuko Seto; Masumi Taguchi; Masanori Ishibashi; Kiyoshi Inoue
Journal:  BMC Microbiol       Date:  2008-06-12       Impact factor: 3.605
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