Mariusz Cierech1, Jacek Wojnarowicz2, Dariusz Szmigiel3, Bohdan Bączkowski1, Anna Maria Grudniak4, Krystyna Izabela Wolska4, Witold Łojkowski2,5, Elżbieta Mierzwińska-Nastalska1. 1. Department of Prosthetic Dentistry, Medical University of Warsaw, Poland. 2. Institute of High Pressure Physic, Polish Academy of Sciences, Warsaw, Poland. 3. Division of Silicon Microsystem and Nanostructure Technology, Institute of Electron Technology, Warsaw, Poland. 4. Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Poland. 5. Faculty of Management, Białystok University of Technology, Białystok, Poland.
Abstract
PURPOSE: The aim of the paper was to investigate the antifungal activity of zinc oxide nanoparticles (ZnONPs) against Candida albicans. Some attempts have been made to find out the best way to introduce ZnONPs into polymethyl methacrylate (PMMA) resin material and to determine some parameters of a newly formed composite. MATERIAL AND METHODS: Zinc oxide nanoparticles were manufactured and their basic physical parameters were determined (average particle size, density, specific surface area). Minimal inhibitory concentration (MIC) of ZnONPs was determined for the Candida albicans standard strain. The average size of ZnO conglomerates in the monomer solution of PMMA resin was measured using a dynamic light scattering instrument. PMMA resin samples with incorporated ZnONPs were produced. The morphology of nanopowder and the newly formed composite was examined under a scanning electron microscope (SEM). In addition, the roughness parameter of PMMA resin material was investigated before and after ZnONPs modification. RESULTS: Nanopowder with the average particle size of 30 nm, density of 5.24 g/cm3 and surface area of 39 m2/g was obtained. MIC was determined at the level of 0.75 mg/mL. The average size of ZnO conglomerates in the monomer solution of acrylic resin dropped by 11 times after ultrasound activation. SEM examination of a newly formed composite showed a successful introduction of ZnONPs confirmed by the energy dispersive X-ray spectroscopy (EDS) analysis. There were no statistically significant differences in the biomaterial roughness before and after the modification of ZnONPs. CONCLUSION: Zinc oxide nanoparticles were successfully incorporated into acrylic resin used for the production of denture bases. The presence of nanoparticles with sizes below 100 nm was confirmed. Nevertheless a newly created composite needs to be further investigated to improve its homogeneity, and to check its microbiological properties, strength and biocompatibility prior to its possible clinical use.
PURPOSE: The aim of the paper was to investigate the antifungal activity of zinc oxide nanoparticles (ZnONPs) against Candida albicans. Some attempts have been made to find out the best way to introduce ZnONPs into polymethyl methacrylate (PMMA) resin material and to determine some parameters of a newly formed composite. MATERIAL AND METHODS:Zinc oxide nanoparticles were manufactured and their basic physical parameters were determined (average particle size, density, specific surface area). Minimal inhibitory concentration (MIC) of ZnONPs was determined for the Candida albicans standard strain. The average size of ZnO conglomerates in the monomer solution of PMMA resin was measured using a dynamic light scattering instrument. PMMA resin samples with incorporated ZnONPs were produced. The morphology of nanopowder and the newly formed composite was examined under a scanning electron microscope (SEM). In addition, the roughness parameter of PMMA resin material was investigated before and after ZnONPs modification. RESULTS: Nanopowder with the average particle size of 30 nm, density of 5.24 g/cm3 and surface area of 39 m2/g was obtained. MIC was determined at the level of 0.75 mg/mL. The average size of ZnO conglomerates in the monomer solution of acrylic resin dropped by 11 times after ultrasound activation. SEM examination of a newly formed composite showed a successful introduction of ZnONPs confirmed by the energy dispersive X-ray spectroscopy (EDS) analysis. There were no statistically significant differences in the biomaterial roughness before and after the modification of ZnONPs. CONCLUSION:Zinc oxide nanoparticles were successfully incorporated into acrylic resin used for the production of denture bases. The presence of nanoparticles with sizes below 100 nm was confirmed. Nevertheless a newly created composite needs to be further investigated to improve its homogeneity, and to check its microbiological properties, strength and biocompatibility prior to its possible clinical use.
Authors: Amanda Aparecida Maia Neves Garcia; Carolina Yoshi Campos Sugio; Lucas José de Azevedo-Silva; Anna Clara Gurgel Gomes; André Ulisses Dantas Batista; Vinicius Carvalho Porto; Simone Soares; Karin Hermana Neppelenbroek Journal: Arch Microbiol Date: 2021-12-24 Impact factor: 2.552
Authors: Maryam Pourhajibagher; Mohammad Noroozian; Mohammad Sadegh Ahmad Akhoundi; Abbas Bahador Journal: BMC Oral Health Date: 2022-05-06 Impact factor: 3.747