Rafal Pokrowiecki1,2,3, Jacek Wojnarowicz4, Tomasz Zareba2, Iwona Koltsov4, Witold Lojkowski4, Stefan Tyski2,5, Agnieszka Mielczarek6, Pawel Zawadzki1. 1. Department of Cranio-Maxillofacial Surgery, Oral Surgery and Implantology, Medical University of Warsaw, Warsaw, Poland. 2. Department of Antibiotics and Microbiology, National Medicines Institute, Warsaw, Poland. 3. Private Practice, Warsaw, Poland. 4. Institute of High Pressure Physics, Polish Academy of Sciences, Warsaw, Poland. 5. Department of Pharmaceutical Microbiology, Medical University of Warsaw, Warsaw, Poland. 6. Department of Conservative Dentistry, Medical University of Warsaw, Warsaw, Poland.
Abstract
AIM: The aims of this study were to investigate new nano-formulations based on ZnO and Ag nanoparticle (NP) compounds when used against clinical strains of oral gram-positive and gram-negative bacteria, and to examine the stability and behaviour of nano-formulation mixtures in saliva based on different compositions of Ag NPs, ZnO NPs and ZnO+x·Ag NPs. Methods: ZnO NPs with and without nanosilver were obtained by microwave solvothermal synthesis. Then, antibacterial activity was evaluated against bacteria isolated from human saliva. Behavior and nanoparticle solutions were evaluated in human saliva and control (artificial saliva and deionized water). Results were statistically compared. RESULTS: The NP mixtures had an average size of 30±3 nm, while the commercial Ag NPs had an average size of 55±5 nm. The suspensions displayed differing antibacterial activities and kinetics of destabilisation processes, depending on NPs composition and fluid types. CONCLUSION: The present study showed that all NPs suspensions displayed significant destabilisation and high destabilisation over the 24 h of the analyses. The agglomeration processes of NPs in human saliva can be reversible.
AIM: The aims of this study were to investigate new nano-formulations based on ZnO and Ag nanoparticle (NP) compounds when used against clinical strains of oral gram-positive and gram-negative bacteria, and to examine the stability and behaviour of nano-formulation mixtures in saliva based on different compositions of Ag NPs, ZnO NPs and ZnO+x·Ag NPs. Methods: ZnO NPs with and without nanosilver were obtained by microwave solvothermal synthesis. Then, antibacterial activity was evaluated against bacteria isolated from human saliva. Behavior and nanoparticle solutions were evaluated in human saliva and control (artificial saliva and deionized water). Results were statistically compared. RESULTS: The NP mixtures had an average size of 30±3 nm, while the commercial Ag NPs had an average size of 55±5 nm. The suspensions displayed differing antibacterial activities and kinetics of destabilisation processes, depending on NPs composition and fluid types. CONCLUSION: The present study showed that all NPs suspensions displayed significant destabilisation and high destabilisation over the 24 h of the analyses. The agglomeration processes of NPs in human saliva can be reversible.
Authors: Anna Salvati; Andrzej S Pitek; Marco P Monopoli; Kanlaya Prapainop; Francesca Baldelli Bombelli; Delyan R Hristov; Philip M Kelly; Christoffer Åberg; Eugene Mahon; Kenneth A Dawson Journal: Nat Nanotechnol Date: 2013-01-20 Impact factor: 39.213
Authors: Gerard H Bode; Karin E Pickl; Maria Sanchez-Purrà; Berta Albaiges; Salvador Borrós; Andy J G Pötgens; Christoph Schmitz; Frank M Sinner; Mario Losen; Harry W M Steinbusch; Hans-Georg Frank; Pilar Martinez-Martinez Journal: PLoS One Date: 2015-05-21 Impact factor: 3.240
Authors: Martin Lundqvist; Cecilia Augustsson; Malin Lilja; Kristoffer Lundkvist; Björn Dahlbäck; Sara Linse; Tommy Cedervall Journal: PLoS One Date: 2017-04-17 Impact factor: 3.240
Authors: Birgit J Teubl; Biljana Stojkovic; Dominic Docter; Elisabeth Pritz; Gerd Leitinger; Igor Poberaj; Ruth Prassl; Roland H Stauber; Eleonore Fröhlich; Johannes G Khinast; Eva Roblegg Journal: Clin Oral Investig Date: 2017-07-09 Impact factor: 3.573
Authors: Sławomir Jaworski; Mateusz Wierzbicki; Ewa Sawosz; Anna Jung; Grzegorz Gielerak; Joanna Biernat; Henryk Jaremek; Witold Łojkowski; Bartosz Woźniak; Jacek Wojnarowicz; Leszek Stobiński; Artur Małolepszy; Marta Mazurkiewicz-Pawlicka; Maciej Łojkowski; Natalia Kurantowicz; André Chwalibog Journal: Nanoscale Res Lett Date: 2018-04-23 Impact factor: 4.703
Authors: C Pushpalatha; Jithya Suresh; V S Gayathri; S V Sowmya; Dominic Augustine; Ahmed Alamoudi; Bassam Zidane; Nassreen Hassan Mohammad Albar; Shankargouda Patil Journal: Front Bioeng Biotechnol Date: 2022-05-19
Authors: Maria Ehsan; Abdul Waheed; Abd Ullah; Abeer Kazmi; Amir Ali; Naveed Iqbal Raja; Zia-Ur-Rehman Mashwani; Tahira Sultana; Nilofar Mustafa; Muhammad Ikram; Huanyong Li Journal: Biomed Res Int Date: 2022-04-30 Impact factor: 3.246
Authors: Rafał Pokrowiecki; Urszula Szałaj; Damian Fudala; Tomasz Zaręba; Jacek Wojnarowicz; Witold Łojkowski; Stefan Tyski; Krzysztof Dowgierd; Agnieszka Mielczarek Journal: Int J Nanomedicine Date: 2022-04-12
Authors: Khaled AbouAitah; Urszula Piotrowska; Jacek Wojnarowicz; Anna Swiderska-Sroda; Ahmed H H El-Desoky; Witold Lojkowski Journal: Int J Mol Sci Date: 2021-05-18 Impact factor: 5.923