BACKGROUND/AIMS: Investigations of Aβ oligomers in neuropathologically confirmed Alzheimer's disease (AD) are still scarce. We report neurohistopathological and biochemical analyses using antibodies against tau and amyloid β (Aβ) pathology. METHODS: Thirty elderly AD patients and 43 age-matched controls with or without deposition of amyloid plaques (AP) were analyzed by immunohistochemistry. In 21 cases with available fresh tissue, Western blots were also performed. Neuropathological analysis included quantitative assessment of neurofibrillary tangles (NFT), AP and Aβ oligomer densities in the mesial temporal cortex (TC). RESULTS: NFT, fibrillar amyloid and Aβ oligomeric deposit densities were significantly higher in AD patients than in controls. There was no relationship between oligomeric Aβ densities and Braak NFT staging scores. Furthermore, Aβ oligomer expression was closely correlated with Aβ plaques in the TC. By Western blot, Aβ oligomers were observed in AD patients, in plaque-free controls, in 1 'tangle-only AD' case, as well as in the cerebellum. A band near 55 kDa was the only Western blot signal that was significantly increased in the TC of AD patients compared to controls as well as less expressed in the cerebellum. CONCLUSION: These results suggest that a putative dodecamer, near 55 kDa, may contribute to AD vulnerability of the TC.
BACKGROUND/AIMS: Investigations of Aβ oligomers in neuropathologically confirmed Alzheimer's disease (AD) are still scarce. We report neurohistopathological and biochemical analyses using antibodies against tau and amyloid β (Aβ) pathology. METHODS: Thirty elderly ADpatients and 43 age-matched controls with or without deposition of amyloid plaques (AP) were analyzed by immunohistochemistry. In 21 cases with available fresh tissue, Western blots were also performed. Neuropathological analysis included quantitative assessment of neurofibrillary tangles (NFT), AP and Aβ oligomer densities in the mesial temporal cortex (TC). RESULTS: NFT, fibrillar amyloid and Aβ oligomeric deposit densities were significantly higher in ADpatients than in controls. There was no relationship between oligomeric Aβ densities and Braak NFT staging scores. Furthermore, Aβ oligomer expression was closely correlated with Aβ plaques in the TC. By Western blot, Aβ oligomers were observed in ADpatients, in plaque-free controls, in 1 'tangle-only AD' case, as well as in the cerebellum. A band near 55 kDa was the only Western blot signal that was significantly increased in the TC of ADpatients compared to controls as well as less expressed in the cerebellum. CONCLUSION: These results suggest that a putative dodecamer, near 55 kDa, may contribute to AD vulnerability of the TC.
Authors: Adriano Sebollela; Erika N Cline; Izolda Popova; Kevin Luo; Xiaoxia Sun; Jay Ahn; Milena A Barcelos; Vanessa N Bezerra; Natalia M Lyra E Silva; Jason Patel; Nathalia R Pinheiro; Lei A Qin; Josette M Kamel; Anthea Weng; Nadia DiNunno; Adrian M Bebenek; Pauline T Velasco; Kirsten L Viola; Pascale N Lacor; Sergio T Ferreira; William L Klein Journal: J Neurochem Date: 2017-08-02 Impact factor: 5.372
Authors: Fatou Amar; Mathew A Sherman; Travis Rush; Megan Larson; Gabriel Boyle; Liu Chang; Jürgen Götz; Alain Buisson; Sylvain E Lesné Journal: Sci Signal Date: 2017-05-09 Impact factor: 8.192
Authors: Marta Querol-Vilaseca; Martí Colom-Cadena; Jordi Pegueroles; Raúl Nuñez-Llaves; Joan Luque-Cabecerans; Laia Muñoz-Llahuna; Jordi Andilla; Olivia Belbin; Tara L Spires-Jones; Ellen Gelpi; Jordi Clarimon; Pablo Loza-Alvarez; Juan Fortea; Alberto Lleó Journal: Sci Rep Date: 2019-03-26 Impact factor: 4.379