Gabriel A Gutiérrez-Rebolledo1, A Georgina Siordia-Reyes2, Mariana Meckes-Fischer3, Adelina Jiménez-Arellanes4. 1. Unidad de Investigación Médica en Farmacología (UIMF), UMAE Hospital de Especialidades, Centro Médico Nacional-Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), México City, Mexico. 2. Servicio de Patología, UMAE Hospital de Pediatría, CMN-SXXI, IMSS, México City, Mexico. 3. Centro de Diagnóstico en Metabolismo Energético y Medicina Mitocondrial, A.C., México City, Mexico. 4. Unidad de Investigación Médica en Farmacología (UIMF), UMAE Hospital de Especialidades, Centro Médico Nacional-Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), México City, Mexico. Electronic address: adelinajim08@prodigy.net.mx.
Abstract
OBJECTIVE: To estimate to what extent the mixture of ursolic acid and oleanolic acid, in addition to the antitubercular standard regime, affects the hepatotoxicity profile. METHODS: Liver injury was induced in male BALB/c mice by administering, per os and daily for 11 weeks, a combination of anti-Tubercular (anti-TB) agents Rifampicin (10 mg/kg), Isoniazid (10 mg/kg), and Pyrazinamide (30 mg/kg). The ursolic acid and oleanolic acid mixture at doses of 100 or 200 μg/mouse/day was subcutaneously injected throughout the entire study period (11 weeks). Biochemical and hematological analysis was supplemented by liver histological examination. RESULTS: Animals treated with the mixture of triterpenic acids exhibited significantly decreased aspartate transaminase and alanine aminotransferase levels and amelioration of the histopathological alterations produced by the anti-TB drugs. CONCLUSIONS: The triterpene mixture was able to prevent the steatosis induced by the anti-TB drugs.
OBJECTIVE: To estimate to what extent the mixture of ursolic acid and oleanolic acid, in addition to the antitubercular standard regime, affects the hepatotoxicity profile. METHODS:Liver injury was induced in male BALB/c mice by administering, per os and daily for 11 weeks, a combination of anti-Tubercular (anti-TB) agents Rifampicin (10 mg/kg), Isoniazid (10 mg/kg), and Pyrazinamide (30 mg/kg). The ursolic acid and oleanolic acid mixture at doses of 100 or 200 μg/mouse/day was subcutaneously injected throughout the entire study period (11 weeks). Biochemical and hematological analysis was supplemented by liver histological examination. RESULTS: Animals treated with the mixture of triterpenic acids exhibited significantly decreased aspartate transaminase and alanine aminotransferase levels and amelioration of the histopathological alterations produced by the anti-TB drugs. CONCLUSIONS: The triterpene mixture was able to prevent the steatosis induced by the anti-TB drugs.
Authors: Patrícia Ferreira Espuri; Larissa Luiza Dos Reis; Eduardo de Figueiredo Peloso; Vanessa Silva Gontijo; Fábio Antônio Colombo; Juliana Barbosa Nunes; Carine Ervolino de Oliveira; Eduardo T De Almeida; Débora E S Silva; Jessica Bortoletto; Daniel Fonseca Segura; Adelino V G Netto; Marcos José Marques Journal: J Biol Inorg Chem Date: 2019-04-04 Impact factor: 3.358