B C MacQueen1, R D Christensen1,2,3, C C Yost1,4, D K Lambert2,5, V L Baer2,5, M J Sheffield2,5, P V Gordon6, M J Cody1, E Gerday2,7, R Schlaberg8,9, J Lowe8, J G Shepherd2,7. 1. Department of Pediatrics, Division of Neonatology, University of Utah School of Medicine, Salt Lake City, UT, USA. 2. Women and Newborn's Clinical Program, Intermountain Healthcare, Salt Lake City, UT, USA. 3. Department of Pediatrics, Division of Hematology/Oncology, University of Utah School of Medicine, Salt Lake City, UT, USA. 4. Program in Molecular Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA. 5. Neonatal Intensive Care, McKay-Dee Hospital, Ogden, UT, USA. 6. Mednax, Sunrise, FL, USA and Sacred Heart Women's and Children's Hospital, Pensacola, FL, USA. 7. Neonatal Intensive Care, Utah Valley Regional Medical Center, Provo, UT, USA. 8. ARUP Laboratories, Salt Lake City, UT, USA. 9. Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA.
Abstract
OBJECTIVE: Neonates with necrotizing enterocolitis (NEC) have higher calprotectin levels in stool than do healthy neonates. However, it is not known whether high stool calprotectin at the onset of bowel symptoms identifies neonates who truly have NEC vs other bowel disorders. STUDY DESIGN: Neonates were eligible for this study when an x-ray was ordered to 'rule-out NEC'. Stool calprotectin was quantified at that time and in a follow-up stool. Each episode was later categorized as NEC or not NEC. The location of calprotectin in the bowel was determined by immunohistochemistry. RESULTS: Neonates with NEC had higher initial and follow-up stool calprotectin levels than did neonates without NEC. Calprotectin in bowel from neonates with NEC was within neutrophil extracellular traps (NETs). CONCLUSION: At the onset of signs concerning for NEC, fecal calprotectin is likely to be higher in neonates with NEC. Calprotectin in their stools is exported from neutrophils via NETs.
OBJECTIVE: Neonates with necrotizing enterocolitis (NEC) have higher calprotectin levels in stool than do healthy neonates. However, it is not known whether high stool calprotectin at the onset of bowel symptoms identifies neonates who truly have NEC vs other bowel disorders. STUDY DESIGN: Neonates were eligible for this study when an x-ray was ordered to 'rule-out NEC'. Stool calprotectin was quantified at that time and in a follow-up stool. Each episode was later categorized as NEC or not NEC. The location of calprotectin in the bowel was determined by immunohistochemistry. RESULTS: Neonates with NEC had higher initial and follow-up stool calprotectin levels than did neonates without NEC. Calprotectin in bowel from neonates with NEC was within neutrophil extracellular traps (NETs). CONCLUSION: At the onset of signs concerning for NEC, fecal calprotectin is likely to be higher in neonates with NEC. Calprotectin in their stools is exported from neutrophils via NETs.
Authors: D K Lambert; R D Christensen; V L Baer; E Henry; P V Gordon; G E Besner; J Wilkes; S E Wiedmeier; E Gerday Journal: J Perinatol Date: 2011-05-12 Impact factor: 2.521
Authors: Kostan W Reisinger; Boris W Kramer; David C Van der Zee; Hens A A Brouwers; Wim A Buurman; Ernest van Heurn; Joep P M Derikx Journal: PLoS One Date: 2014-03-06 Impact factor: 3.240
Authors: Qinjie Zhou; Diego F Niño; Yukihiro Yamaguchi; Sanxia Wang; William B Fulton; Hongpeng Jia; Peng Lu; Thomas Prindle; David Pamies; Meaghan Morris; Liam L Chen; Chhinder P Sodhi; David J Hackam Journal: Sci Transl Med Date: 2021-01-06 Impact factor: 17.956
Authors: Jelena R Cekovic; Nikola S Prodanovic; Sara S Mijailovic; Sanja M Knezevic; Biljana P Vuletic; Andjelka K Stojkovic; Dragana M Savic; Tijana V Prodanovic; Marina M Stanojevic; Aleksandra M Simovic Journal: Open Med (Wars) Date: 2022-07-13
Authors: Oluwabunmi O Olaloye; Peng Liu; Jessica M Toothaker; Blake T McCourt; Collin C McCourt; Jenny Xiao; Erica Prochaska; Spenser Shaffer; Lael Werner; Jordan Gringauz; Misty Good; Jeffrey D Goldsmith; Xiaojing An; Fujing Wang; Scott B Snapper; Dror Shouval; Kong Chen; George Tseng; Liza Konnikova Journal: J Exp Med Date: 2021-07-16 Impact factor: 14.307
Authors: Aifeng Lin; Robert D Inman; Catherine J Streutker; Zhenbo Zhang; Kenneth P H Pritzker; Hing Wo Tsui; Florence W L Tsui Journal: Arthritis Res Ther Date: 2020-03-18 Impact factor: 5.156
Authors: Hala Chaaban; Kathryn Burge; Jeffrey Eckert; Ravi S Keshari; Robert Silasi; Cristina Lupu; Barbara Warner; Marilyn Escobedo; Michael Caplan; Florea Lupu Journal: J Cell Mol Med Date: 2020-06-08 Impact factor: 5.310