Alona Bin-Nun1, Christina Booms2, Naama Sabag3, Refael Mevorach3, Nurit Algur3, Cathy Hammerman1. 1. Department of Neonatology, Shaare Zedek Medical Center, Jerusalem, Israel. 2. Department of Neonatology, Hadassah Hebrew University Medical Center, Jerusalem, Israel. 3. Biochemistry Laboratory, Shaare Zedek Medical Center, Jerusalem, Israel.
Abstract
OBJECTIVE: The aim of this study is to compare fecal calprotectin (FC) levels as measured by a rapid FC assay with those measured by enzyme-linked immunosorbent assay (ELISA) from concurrent stool samples. We also attempted to demonstrate a correlation between elevated rapid assay FC levels and the presence of necrotizing enterocolitis (NEC) and to define a cutoff FC value which could serve as a basis for diagnosing NEC in the future. STUDY DESIGN: Stool samples were collected for FC analysis at 1 and 3 weeks postnatally and whenever there was clinical suspicion of NEC. RESULTS: Rapid assay FC levels were elevated with NEC (3,000 µg/g stool [2075,7875] vs. without (195 µg/g stool [110,440] p < 0.001); and were well correlated with ELISA FC levels (r(2) = 0.89). CONCLUSION: We present the first data showing that rapid assay FC levels are potentially useful in the bedside diagnosis of NEC. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.
OBJECTIVE: The aim of this study is to compare fecal calprotectin (FC) levels as measured by a rapid FC assay with those measured by enzyme-linked immunosorbent assay (ELISA) from concurrent stool samples. We also attempted to demonstrate a correlation between elevated rapid assay FC levels and the presence of necrotizing enterocolitis (NEC) and to define a cutoff FC value which could serve as a basis for diagnosing NEC in the future. STUDY DESIGN: Stool samples were collected for FC analysis at 1 and 3 weeks postnatally and whenever there was clinical suspicion of NEC. RESULTS: Rapid assay FC levels were elevated with NEC (3,000 µg/g stool [2075,7875] vs. without (195 µg/g stool [110,440] p < 0.001); and were well correlated with ELISA FC levels (r(2) = 0.89). CONCLUSION: We present the first data showing that rapid assay FC levels are potentially useful in the bedside diagnosis of NEC. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.
Authors: B C MacQueen; R D Christensen; C C Yost; D K Lambert; V L Baer; M J Sheffield; P V Gordon; M J Cody; E Gerday; R Schlaberg; J Lowe; J G Shepherd Journal: J Perinatol Date: 2016-07-07 Impact factor: 2.521
Authors: Ferdinando D'Amico; David T Rubin; Paulo Gustavo Kotze; Fernando Magro; Britta Siegmund; Taku Kobayashi; Pablo A Olivera; Peter Bossuyt; Lieven Pouillon; Edouard Louis; Eugeni Domènech; Subrata Ghosh; Silvio Danese; Laurent Peyrin-Biroulet Journal: United European Gastroenterol J Date: 2021-05-07 Impact factor: 4.623