| Literature DB >> 27376835 |
Yongxiang Jiang1, Bao Tang1, Zongqi Xu1, Kun Liu1, Zheng Xu1, Xiaohai Feng2, Hong Xu1.
Abstract
The production of poly-γ-glutamic acid (γ-PGA) by Bacillus subtilis NX-2 using a moving bed biofilm reactor (MBBR) system was tested for the first time in this study. Polypropylene TL-2 was chosen as a suitable carrier, and γ-PGA concentration of 42.7±0.86g/L and productivity of 0.59±0.06g/(Lh) were obtained in batch fermentation. After application of the strategy of dissolved oxygen (DO)-stat feeding, higher γ-PGA concentration and productivity were achieved than with glucose feedback feeding. Finally, the repeated fed-batch cultures implemented in the MBBR system showed high stability, and the maximal γ-PGA concentration and productivity of 74.2g/L and 1.24g/(Lh) were achieved, respectively. In addition, the promotion of oxygen transfer by an MBBR carrier was well explained by a computational fluid dynamics (CFD) simulation. These results suggest that an MBBR system could be applied to large-scale γ-PGA production.Entities:
Keywords: Bacillus subtilis NX-2; CFD simulation; DO-stat; Moving bed biofilm reactor; Poly(γ-glutamic acid)
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Year: 2016 PMID： 27376835 DOI： 10.1016/j.biortech.2016.06.103
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642