Andrew J Belli1, Sonali Bose1, Neil Aggarwal1, Christopher DaSilva1, Sritika Thapa1, Laura Grammer1, Laura M Paulin1, Nadia N Hansel2. 1. Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, United States. 2. Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, United States; Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, United States. Electronic address: nhansel1@jhmi.edu.
Abstract
INTRODUCTION: Exposure to fine particulate matter (PM2.5) is associated with worse morbidity in individuals with COPD. Inhaled PM is phagocytosed by airway macrophages (AM), and black carbon measured in AM may serve as a biomarker of air pollution exposure. As there is little data on how indoor PM exposure may influence AM black carbon content in those with respiratory disease, we investigated the association of indoor PM2.5 concentration to AM black carbon content in adults with COPD. METHODS: Former smokers (>10 pack-years smoking history, quit date >1 year prior to enrollment) older than 40 years of age with moderate-severe COPD were eligible. Indoor air PM2.5 concentrations were measured over 5-7 days at baseline, 3 month, and 6 month intervals. Sputum induction was performed during clinic visits concordant with home monitoring. A total of 50 macrophages per sputum specimen were photographed and quantified using appropriate software by trained staff blinded to PM concentrations. Longitudinal analyses using generalized estimating equations were used to assess the relationship between indoor PM exposure and AM black carbon content. RESULTS: Participants (n=20) were older (mean (SD) age 67 (4) years), predominantly Caucasian (85%) and male (70%), with an average smoking history of 52 pack-years and mean (SD) quit date of 13 (9) years prior to enrollment. The majority of daily time was reported to be spent indoors (>23h). Mean indoor PM2.5 concentration was 12.8 (13.5)µg/m(3). The mean area of black carbon quantified in airway macrophages was 1.2 (0.7)µm(2). In multivariate cross-sectional and longitudinal analyses, each 10µg/m(3) increase in indoor PM2.5 was significantly associated with a 0.26µm(2) and 0.19µm(2) increase in airway macrophage black carbon total area, respectively (p<0.05). CONCLUSION: Higher indoor PM2.5 concentration is associated with an increase in black carbon content of AM in individuals with COPD. These data support the potential for AM black carbon content to be a useful non-invasive biomarker of exposure to indoor PM.
INTRODUCTION: Exposure to fine particulate matter (PM2.5) is associated with worse morbidity in individuals with COPD. Inhaled PM is phagocytosed by airway macrophages (AM), and black carbon measured in AM may serve as a biomarker of air pollution exposure. As there is little data on how indoor PM exposure may influence AM black carbon content in those with respiratory disease, we investigated the association of indoor PM2.5 concentration to AM black carbon content in adults with COPD. METHODS: Former smokers (>10 pack-years smoking history, quit date >1 year prior to enrollment) older than 40 years of age with moderate-severe COPD were eligible. Indoor air PM2.5 concentrations were measured over 5-7 days at baseline, 3 month, and 6 month intervals. Sputum induction was performed during clinic visits concordant with home monitoring. A total of 50 macrophages per sputum specimen were photographed and quantified using appropriate software by trained staff blinded to PM concentrations. Longitudinal analyses using generalized estimating equations were used to assess the relationship between indoor PM exposure and AM black carbon content. RESULTS:Participants (n=20) were older (mean (SD) age 67 (4) years), predominantly Caucasian (85%) and male (70%), with an average smoking history of 52 pack-years and mean (SD) quit date of 13 (9) years prior to enrollment. The majority of daily time was reported to be spent indoors (>23h). Mean indoor PM2.5 concentration was 12.8 (13.5)µg/m(3). The mean area of black carbon quantified in airway macrophages was 1.2 (0.7)µm(2). In multivariate cross-sectional and longitudinal analyses, each 10µg/m(3) increase in indoor PM2.5 was significantly associated with a 0.26µm(2) and 0.19µm(2) increase in airway macrophage black carbon total area, respectively (p<0.05). CONCLUSION: Higher indoor PM2.5 concentration is associated with an increase in black carbon content of AM in individuals with COPD. These data support the potential for AM black carbon content to be a useful non-invasive biomarker of exposure to indoor PM.
Authors: M R Miller; J Hankinson; V Brusasco; F Burgos; R Casaburi; A Coates; R Crapo; P Enright; C P M van der Grinten; P Gustafsson; R Jensen; D C Johnson; N MacIntyre; R McKay; D Navajas; O F Pedersen; R Pellegrino; G Viegi; J Wanger Journal: Eur Respir J Date: 2005-08 Impact factor: 16.671
Authors: N E Klepeis; W C Nelson; W R Ott; J P Robinson; A M Tsang; P Switzer; J V Behar; S C Hern; W H Engelmann Journal: J Expo Anal Environ Epidemiol Date: 2001 May-Jun
Authors: Neeta S Kulkarni; Benjamin Prudon; Sri L Panditi; Yekoye Abebe; Jonathan Grigg Journal: Sci Total Environ Date: 2004-12-21 Impact factor: 7.963
Authors: Rossa E Brugha; Naseem Mushtaq; Thomas Round; Dev H Gadhvi; Isobel Dundas; Erol Gaillard; Lee Koh; Louise J Fleming; Daniel J Lewis; Marek Sanak; Helen E Wood; Benjamin Barratt; Ian S Mudway; Frank J Kelly; Christopher J Griffiths; Jonathan Grigg Journal: Thorax Date: 2014-02-24 Impact factor: 9.139
Authors: Nadia N Hansel; Meredith C McCormack; Andrew J Belli; Elizabeth C Matsui; Roger D Peng; Charles Aloe; Laura Paulin; D'Ann L Williams; Gregory B Diette; Patrick N Breysse Journal: Am J Respir Crit Care Med Date: 2013-05-15 Impact factor: 21.405
Authors: Francesca Dominici; Roger D Peng; Michelle L Bell; Luu Pham; Aidan McDermott; Scott L Zeger; Jonathan M Samet Journal: JAMA Date: 2006-03-08 Impact factor: 56.272
Authors: Marina Zusman; Amanda J Gassett; Kipruto Kirwa; R Graham Barr; Christopher B Cooper; MeiLan K Han; Richard E Kanner; Kirsten Koehler; Victor E Ortega; Robert Paine Rd; Laura Paulin; Cheryl Pirozzi; Ana Rule; Nadia N Hansel; Joel D Kaufman Journal: Indoor Air Date: 2020-12-28 Impact factor: 6.554
Authors: Nadia N Hansel; Laura M Paulin; Amanda J Gassett; Roger D Peng; Neil Alexis; Vincent S Fan; Eugene Bleecker; Russell Bowler; Alejandro P Comellas; Mark Dransfield; MeiLan K Han; Victor Kim; Jerry A Krishnan; Cheryl Pirozzi; Christopher B Cooper; Fernando Martinez; Prescott G Woodruff; Patrick J Breysse; R Graham Barr; Joel D Kaufman Journal: BMJ Open Respir Res Date: 2017-05-18
Authors: Vickram Tejwani; Eric Moughames; Karthik Suresh; Shih-En Tang; Laura G Mair; Karina Romero; Nirupama Putcha; Neil E Alexis; Han Woo; Franco R D'Alessio; Nadia N Hansel Journal: Chronic Obstr Pulm Dis Date: 2021-01