David Briskey1, Mandy Heritage2, Lesley-Anne Jaskowski2, Jonathan Peake3, Glenda Gobe4, V Nathan Subramaniam5, Darrell Crawford2, Catherine Campbell6, Luis Vitetta7. 1. School of Human Movement and Nutrition Sciences, The University of Queensland, Brisbane, Queensland, Australia. 2. Gallipoli Medical Research Centre, Greenslopes Hospital, Brisbane, Australia School of Medicine, The University of Queensland, Brisbane, Australia. 3. School of Biomedical Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia. 4. Centre for Kidney Disease Research, School of Medicine, The University of Queensland, Translational Research Institute, Queensland, Australia. 5. Gallipoli Medical Research Centre, Greenslopes Hospital, Brisbane, Australia Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Australia Envoi Specialist Pathologists, Brisbane, Australia. 6. Envoi Specialist Pathologists, Brisbane, Australia. 7. Sydney Medical School, University of Sydney, Medlab Clinical Ltd., Sydney, New South Wales, 2006, Australia.
Abstract
BACKGROUND: We have investigated the effects of a multispecies probiotic preparation containing a combination of probiotic bacterial genera that included Bifidobacteria, Lactobacilli and a Streptococcus in a mouse model of high-fat diet or obesity-induced liver steatosis. METHODS: Three groups of C57B1/6J mice were fed either a standard chow or a high-fat diet for 20 weeks, while a third group was fed a high-fat diet for 10 weeks and then concomitantly administered probiotics for a further 10 weeks. Serum, liver and large bowel samples were collected for analysis. RESULTS: The expression of the tight-junction proteins ZO-1 and ZO-2 was reduced (p < 0.05) in high-fat diet-fed mice compared to chow-fed mice. Probiotic supplementation helped to maintain tight ZO-1 and ZO-2 expression compared with the high-fat diet group (p < 0.05), but did not restore ZO-1 or ZO-2 expression compared with chow-fed mice. Mice fed a high-fat diet ± probiotics had significant steatosis development compared with chow-fed mice (p < 0.05); steatosis was less severe in the probiotics group compared with the high-fat diet group. Hepatic triglyceride concentration was higher in mice fed a high-fat diet ± probiotics compared with the chow group (p < 0.05), and was lower in the probiotics group compared with the high-fat diet group (p < 0.05). Compared with chow-fed mice, serum glucose, cholesterol concentration and the activity of alanine transaminase were higher (p < 0.05), whereas serum triglyceride concentration was lower (p < 0.05) in mice fed a high-fat diet ± probiotics. CONCLUSIONS: Supplementation with a multispecies probiotic formulation helped to maintain tight-junction proteins ZO-1 and ZO-2, and reduced hepatic triglyceride concentration compared with a high-fat diet alone.
BACKGROUND: We have investigated the effects of a multispecies probiotic preparation containing a combination of probiotic bacterial genera that included Bifidobacteria, Lactobacilli and a Streptococcus in a mouse model of high-fat diet or obesity-induced liver steatosis. METHODS: Three groups of C57B1/6J mice were fed either a standard chow or a high-fat diet for 20 weeks, while a third group was fed a high-fat diet for 10 weeks and then concomitantly administered probiotics for a further 10 weeks. Serum, liver and large bowel samples were collected for analysis. RESULTS: The expression of the tight-junction proteins ZO-1 and ZO-2 was reduced (p < 0.05) in high-fat diet-fed mice compared to chow-fed mice. Probiotic supplementation helped to maintain tight ZO-1 and ZO-2 expression compared with the high-fat diet group (p < 0.05), but did not restore ZO-1 or ZO-2 expression compared with chow-fed mice. Mice fed a high-fat diet ± probiotics had significant steatosis development compared with chow-fed mice (p < 0.05); steatosis was less severe in the probiotics group compared with the high-fat diet group. Hepatic triglyceride concentration was higher in mice fed a high-fat diet ± probiotics compared with the chow group (p < 0.05), and was lower in the probiotics group compared with the high-fat diet group (p < 0.05). Compared with chow-fed mice, serum glucose, cholesterol concentration and the activity of alanine transaminase were higher (p < 0.05), whereas serum triglyceride concentration was lower (p < 0.05) in mice fed a high-fat diet ± probiotics. CONCLUSIONS: Supplementation with a multispecies probiotic formulation helped to maintain tight-junction proteins ZO-1 and ZO-2, and reduced hepatic triglyceride concentration compared with a high-fat diet alone.
Authors: R Aller; D A De Luis; O Izaola; R Conde; M Gonzalez Sagrado; D Primo; B De La Fuente; J Gonzalez Journal: Eur Rev Med Pharmacol Sci Date: 2011-09 Impact factor: 3.507
Authors: Helen Waller-Evans; Christophe Hue; Jane Fearnside; Alice R Rothwell; Helen E Lockstone; Sophie Caldérari; Steven P Wilder; Jean-Baptiste Cazier; James Scott; Dominique Gauguier Journal: PLoS One Date: 2013-12-06 Impact factor: 3.240