Lilla Landeck1,2, Ivone Jakasa3, Irena Dapic3, René Lutter4, Jacob P Thyssen5, Lone Skov5, Andrea Braun6,7, Michael P Schön6,7, Swen M John1,7, Sanja Kezic8, Richard Brans1,7. 1. Department of Dermatology, Environmental Medicine and Health Theory, University of Osnabrück, Osnabrück, Germany. 2. Department of Dermatology, Ernst von Bergmann General Hospital, Teaching Hospital Charité University Medicine Berlin, Potsdam, Germany. 3. Laboratory for Analytical Chemistry, Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia. 4. Departments of Respiratory Medicine and Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. 5. Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark. 6. Department of Dermatology, Venereology, and Allergology, University Medical Center Göttingen, Georg August University, Göttingen, Germany. 7. Lower Saxony Institute of Occupational Dermatology, University of Osnabrück and University Medical Center Göttingen, Osnabrück/Göttingen, Germany. 8. Coronel Institute for Occupational Health, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Abstract
BACKGROUND/ PURPOSE: Urocanic acid (UCA) absorbs ultraviolet (UV)B radiation in the epidermis which may interfere with phototherapy. Therefore, the influence of individual levels of UCA on immune reactivity and vitamin D synthesis induced by narrowband UVB radiation was assessed. METHODS: Twenty-eight subjects with irritant contact dermatitis of the hands were irradiated with suberythemal doses of narrowband UVB radiation on their unaffected lower forearms on three consecutive days. Stratum corneum tape strips and epidermal interstitial fluid (ISF) as well as blood samples were analyzed. RESULTS: Narrowband UVB irradiation led to the conversion of trans-UCA into its cis-isomer in the epidermis. The observed increase in 25-hydroxyvitamin D serum concentrations was inversely correlated with the baseline levels of trans-UCA. Furthermore, UVB irradiation induced significant changes in the levels of CXCL10/IP-10, CCL2/MCP-1, CCL4/MIP-1β, and the IL-1RA/IL-1α ratio. The levels of IL-1α and CXCL9/MIG showed a trend toward increase. The changes in the levels of inflammatory and immunomodulatory mediators did not depend on baseline levels of trans-UCA. CONCLUSION: The results suggest that epidermal levels of trans-UCA affect vitamin D synthesis, but not cutaneous immune reactivity upon repeated exposure to suberythemal doses of narrowband UVB radiation. However, this requires further exploration.
BACKGROUND/ PURPOSE:Urocanic acid (UCA) absorbs ultraviolet (UV)B radiation in the epidermis which may interfere with phototherapy. Therefore, the influence of individual levels of UCA on immune reactivity and vitamin D synthesis induced by narrowband UVB radiation was assessed. METHODS: Twenty-eight subjects with irritant contact dermatitis of the hands were irradiated with suberythemal doses of narrowband UVB radiation on their unaffected lower forearms on three consecutive days. Stratum corneum tape strips and epidermal interstitial fluid (ISF) as well as blood samples were analyzed. RESULTS: Narrowband UVB irradiation led to the conversion of trans-UCA into its cis-isomer in the epidermis. The observed increase in 25-hydroxyvitamin D serum concentrations was inversely correlated with the baseline levels of trans-UCA. Furthermore, UVB irradiation induced significant changes in the levels of CXCL10/IP-10, CCL2/MCP-1, CCL4/MIP-1β, and the IL-1RA/IL-1α ratio. The levels of IL-1α and CXCL9/MIG showed a trend toward increase. The changes in the levels of inflammatory and immunomodulatory mediators did not depend on baseline levels of trans-UCA. CONCLUSION: The results suggest that epidermal levels of trans-UCA affect vitamin D synthesis, but not cutaneous immune reactivity upon repeated exposure to suberythemal doses of narrowband UVB radiation. However, this requires further exploration.
Authors: Rhonda M Brand; Peter Wipf; Austin Durham; Michael W Epperly; Joel S Greenberger; Louis D Falo Journal: Front Pharmacol Date: 2018-08-20 Impact factor: 5.810
Authors: Anne J Keurentjes; Kornelis D de Witt; Ivone Jakasa; Lars Rüther; Patrick M J H Kemperman; Sanja Kezic; Christoph Riethmüller Journal: Exp Dermatol Date: 2020-03-13 Impact factor: 3.960
Authors: Naomi R Wray; John J McGrath; Joana A Revez; Tian Lin; Zhen Qiao; Angli Xue; Yan Holtz; Zhihong Zhu; Jian Zeng; Huanwei Wang; Julia Sidorenko; Kathryn E Kemper; Anna A E Vinkhuyzen; Julanne Frater; Darryl Eyles; Thomas H J Burne; Brittany Mitchell; Nicholas G Martin; Gu Zhu; Peter M Visscher; Jian Yang Journal: Nat Commun Date: 2020-04-02 Impact factor: 14.919