Vector competence of Aedes aegypti in transmitting Chikungunya virus: effects and implications of extrinsic incubation temperature on dissemination and infection rates.

BACKGROUND: Aedes aegypti is a competent arthropod vector of chikungunya virus (CHIKV). The rate at which the virus disseminate in the vector is limited by temperature of their environment which can be an important determinant of geographical and seasonal limits to transmission by the arthropods in the tropics. This study investigated the vector competence of Ae. aegypti for CHIKV at ambient temperature of 32 and 26 °C (Coastal and Western Kenya respectively) reared at Extrinsic Incubation Temperature (EIT) of 32 and 26 °C that resembles those in the two regions.
METHODS: Ae. aegypti eggs were collected from coastal and Western Kenya, hatched in the insectary and reared to F1 generation. Four-day old mosquitoes were exposed to CHIKV through a membrane feeding. They were then incubated in temperatures mimicking the mean annual temperatures for Trans-Nzoia (26 °C) and Lamu (32 °C). After every 7, 10 and 13 days post infection (DPI); one third of exposed mosquitoes were sampled and assayed for virus infection and dissemination.
RESULTS: The midgut infection rates (MIR) of Ae. aegypti sampled from Coastal Region was significantly (p < 0.05) higher than those sampled from Western Kenya, with no statistical differences observed for the coastal Ae. aegypti at EIT 26 and at 32 °C. The MIR of Ae. aegypti from the Western Region was significantly (p < 0.05) affected by the EIT, with mosquito reared at EIT 32 °C exhibiting higher MIR than those reared at EIT 26 °C. There was a significant (p < 0.05) interactive effects of the region, EIT and DPI on MIR. The disseminated infection rates for the CHIKV in Ae. aegypti in the legs (DIR-L) was higher in mosquitoes sampled from Coast regardless of the EIT while those from Western Kenya, dissemination rates were significantly higher at higher EIT of 32 °C.
CONCLUSIONS: Vector competence was higher in mosquito populations reared under high temperatures which weakens the midgut infection barrier. Hence, suggesting Lamu population is more susceptible to CHIKV therefore having a weaker mid gut infection barrier than the Trans Nzoia population. These underscores importance of examining the course of infection at various ambient temperatures and EIT between regions mosquito populations.

Background

Chikungunya fever is a self-remitting febrile viral illness caused by Chikungunya virus (CHIKV). The CHIKV is an arthropod-borne virus (arbovirus) of alphavirus genus in the family Togaviridae. The term “Chikungunya” was derived from the African dialect Swahili or Makonde and translates as “to be bent over and refers to the “stooped-over posture” exhibited by individuals with the disease [1]. The roots of this viral illness date back to 1953, when it was first detected in a Makonde Village in the Newala District of Tanzania [2, 3]. CHIKV infection is usually characterized by an acute onset of fever, rash, and arthralgias, and is often accompanied by headache, joint swelling and conjunctivitis [4-8]. Chikungunya disease is rarely fatal but is associated with significant morbidity. Although frequent outbreaks have been reported in the tropical countries of Africa and Southeast Asia, there are recent concern in Western countries and temperate zones around the world [9, 10]. In Africa, high prevalence of the CHIKV has been reported with first case being isolated in Tanzania in 1953 [1, 2], Union of the Comoros in 2005 [11], Congo (DRC) during 1998–2000 [12, 13], Central African Republic in 1999–2000 [14] and Mauritius and Madagascar in 2005 and 2006 respectively [15]. The dynamics attest to overall varying outbreak trends being observed in East/South/Central Africa and western Africa countries [11]. Kenya has experienced two outbreaks of chikungunya fever in 2004 [16] with the latest outbreak occurring in May 2016 in Northern Kenya (see Additional file 1) due to close proximity of mosquito breeding sites to human habitation and heavy rainfall [17]. Large variations in prevalence within these countries have also been reported such as the 59 % seroprevalence of the CHIKV infection in Busia District and 24 % in Malindi in Kenya [18].

The vectors principally responsible for transmission of the virus are Aedes mosquitoes [19, 20] where the virus actively replicates but the viral transmission occurs through the mosquitoes involved if the virus overcomes a series of anatomical barriers, i.e. the midgut and the salivary glands. In the past, large epidemics were related to the presence of the primary vector Ae. aegypti, which is also the main vector of the dengue virus [6, 21, 22]. Ae. aegypti was established in southern parts of continental Europe until the mid-1900s but subsequently disappeared for reasons that are yet to be completely understood [21]. In Africa, CHIKV apparently is maintained in a sylvatic transmission cycle involving primates and forest-dwelling Aedes mosquitoes [23]. Sylvatic vectors that have been implicated in transmission include Ae. africanus and Ae. aegypti in East Africa [24, 25]. Ae. aegypti predominantly breeds in stored fresh water, such as desert coolers, flower vases, water-tanks, etc., and in peri-domestic areas (discarded household junk items like vehicular tyres, coconut shells, pots, cans, bins, etc.) in urban and semi urban environments [26, 27]. Adult mosquitoes rest in cool and shady areas and bite humans during the daytime.

In mosquito infected by CHIKV, the extrinsic incubation period (EIP), the time from initial acquisition of pathogens until transmission is possible [28, 29], ranges from 2 to 9 days, with an average of 3 days [30, 31]. CHIKV is transmitted by Aedes mosquitoes, mainly by Ae. aegypti. The Ae. aegypti, is well distributed and is highly anthropophilic [32-34], thus increases the risk of CHIKV transmission. Mosquito vectors display different degrees of vector competence for different CHIKV isolates [35]. However, the invasive species Ae. albopictus has played a major role in most of recent epidemics since its last emergence in Kenya in 2004 [34, 36, 37]. Furthermore, recent studies have shown that transmission and spread of CHIKV in Africa and Asia is related to the CHIKV phylogroup and mosquito species [11, 14, 38, 39]. In the present study, CHIKV strain isolated from the 2004–2005 outbreak in Lamu Island was considered, the East/South/Central Africa and Indian Ocean genotype [14, 38]. Although the establishment of an arbovirus infection in a mosquito following ingestion of a virus is dependent on the amount of viral particles ingested by the mosquito and the susceptibility of the mosquito to infection by the virus [40], the vector competence is a complex trait involving an interplay between vectors, pathogens and environmental factors [35, 41, 42]. Temperature is regarded as one of the most important abiotic environmental factors affecting biological processes of mosquitoes, including interactions with arboviruses. Seasonal and geographic differences in temperature and anticipated climate change undoubtedly influence mosquito population dynamics, individuals’ traits related to vector biology (lifespan and vector competence for arboviruses), and disease transmission patterns. Extrinsic incubation temperature (EIT) has been shown to influence the replication and dissemination of arboviruses in vectors [43] thus altering the Extrinsic Incubation Period (EIP) [28, 29]. In the tropics, areas of high prevalence of the mosquitoes, with reported occurrence of CHIKV have variable temperature ranging between 25 and 34 °C throughout the year as part of climate characteristics. Yet information on the vector transmission of different populations of this species for CHIKV at different EIT is limited. Therefore the aim of this study was to compare the vector competence of coastal and Western Kenya Ae. aegypti populations for CHIKV under varying EIT. The coastal and western regions of Kenya have mean annual ambient temperature of 32 and 26 °C respectively. The information generated from this study provides data on competence factors that would influence epidemiological patterns of chikungunya fever.

Methods

Study setting

This study was conducted in Western Kenya and at the Coast. In Western Kenya, samples were obtained from Kiminini and Sasuri village in Trans Nzoia County while Lamu and Shela Village in Lamu County represented the Coastal region. Trans Nzoia has a latitude of 1.0567° N, and a longitude of 34.9507° E and a temperature range between 10 and 27 °C with an annual precipitation ranging between 1000 and 1200 mm, with the wettest months being experienced between April and October. The elevation of Kitale is about 1900 m. Meanwhile, Lamu County is situated in Kenya’s former Coast Province, at a longitude of 040°S´E and a latitude of 02°17´S, and is headquartered in Lamu town. The county covers a strip of northeastern coastal mainland and the Lamu Archipelago. It has a population of 101,539, and its land area is 6,167 km2. Lamu has a tropical savannah climate with average annual temperatures ranging between 13.6 and 40.9 °C. The county receives annual precipitation ranging between 900 and 1100 mm, with the rainy season experienced between May and June. From July the environment gradually gets hotter and dryer until March/April when it reaches 40 °C. Shela Village on Lamu Island is a tangle of narrow sandy lanes some smaller thatched dwellings and mosques. It is about 3.2 km south of Lamu Town. An additional file shows Map of the study areas in more detail (see Additional file 2).

Mosquito eggs collection

Eggs of Ae. aegypti were collected using oviposition traps that consisted of black plastic cups lined with oviposition paper and half-filled with water. A total of 25 ovitraps were placed at least 50 m apart in each of the study setting. These ovitraps with oviposition papers were left in the peridomestic areas for four days. On the 4th day, all the ovicups were collected and the eggs transported to the biosafety level-2 (BSL-2) laboratory where they were dried on damp cotton wool to quiescent state as earlier described [44, 45] and stored in an air tight container at room temperature in the insectary.

Mosquito rearing

Mosquitoes were reared in the insectary, maintained at a temperature of 28 °C and 80 % relative humidity (RH), with a 12:12-h (Light:Dark) photoperiod. The eggs were hatched and the larvae fed on TetraMin® fish food until pupation. Pupae were transferred to small plastic cups half-filled with water, placed in 4-l plastic cages screened with netting material on top and allowed to emerge into F0 adult mosquitoes. The emerging adults were then morphologically identified under a dissecting microscope using taxonomic keys of Edwards [46], to confirm that only Ae. aegypti mosquitoes were used in the subsequent experiments. The identified female Ae. aegypti were returned to experimental cages, blood fed on clean laboratory-bred mice and provided with oviposition papers to lay F1 eggs. The F1 eggs were hatched and reared as outlined and only adult female mosquitoes were used in the succeeding experiments [47].

Preparation of virus stock

CHIKV strain isolated from the 2004–2005 outbreak in Lamu Island (Lamu001) was used in this study, the East/South/Central Africa and Indian Ocean genotype (group III), subgroup: East/South/Central African subgroup (IIIa and b) [14, 38]. The working stock virus was prepared by propagating CHIKV in Vero cells in T25 culture flasks. The infected cells were incubated at 37 °C and 5 % CO2 and observed daily for cytopathic changes before the virus was harvested. The virus stock titer was determined [log10 7.2 plaque-forming units (pfu)/mL] by plaque assay and aliquoted in cryovials and stored at −80 °C until usage [48].

Oral infection of mosquitoes with the virus

Infection was performed in BSL2 insectary using four-day old mosquitoes after they were deprived of sucrose solution and water 24 h prior to exposure to the infectious blood meal using a membrane feeding apparatus [49]. A Hemotek membrane-feeding system (Discovery Workshops, Accrington, U.K.) which employed an electric heating element to maintain the temperature of the blood meal constant at 37 °C, was used. The infectious blood meal (log10 5.9 pfu/mL) was prepared by mixing 500 μl of the working virus with 500 μl of defribrinated sheep blood. The blood-virus mixture was pipetted into each membrane unit which was screwed onto the heating chamber and placed on top of mosquito cages each containing between 50–350 mosquitoes which were allowed to feed for 45 min [50-52]. Fully engorged mosquitoes were aspirated and transferred to empty 4-l plastic cages and maintained on 10 % glucose solution. These mosquitoes were reared for up to 13 days at low and high EITs of 26 °C or 32 °C respectively, mimicking the mean annual temperatures for Trans-Nzoia and Lamu counties respectively.

Test for infection and dissemination rates of CHIKV

Mosquito sampling and dissections

A third of the fed mosquitoes were randomly sampled on day 7, 10, 13 post exposure (pe) and were dissected into abdomen, head and legs [25, 28, 53–55]. The abdomens of each mosquito were individually triturated in 1 mL of Eagle’s Minimum Essential Medium (MEM) (Sigma Aldrich) diluents and frozen at −80 °C until assayed for CHIKV by plaque assay on Vero cell monolayers. The dilutions were inoculated on confluent Vero cell monolayers in 12-well plates. Each plate was labeled with sample and dilution descriptions and for each virus stock serial dilutions (100, 10−1, 10−2, 10−3, and 10−4) were made.

Virus assay in mosquito bodies

To demonstrate virus infection, 10 fold serial dilutions of the abdominal homogenates were made in Minimum Essential Medium Eagle (MEM), with Earle’s salts and reduced NaHCO3 (Sigma- Aldrich, St. Louis, MO) supplemented with 15 % FBS (Sigma-Aldrich), 2 % L-glutamine (Sigma- Aldrich) and 2 % antibiotic/antimycotic solution (Sigma-Aldrich) with 10,000 μl penicillin, 10 mg streptomycin and 25 μg amphotericin B per milliliter and tested for the presence of virus on Vero cell monolayers, by plaque assay, in 12-well plates. One hundred microliters of the appropriate dilutions of the abdominal homogenates was added to each of ten wells of the 12-well plate to infect the cells and the remaining two wells were used for controls. This was repeated to all the corresponding plates and dilutions. The plates were incubated at 37 °C and gently rocked every 15 min for 1 h to allow for virus adsorption. One milliliter of methyl cellulose solution (1.25 % Methylcellulose in MEM with 2 % FBS) was added gently to each well, and the plates incubated at 37 °C in a humidified 5 % CO2 incubator for 3 days. On the 3rd day, methylcellulose was removed from the wells using disposable plastic pipette in bio-safety cabinet. The plates were fixed by adding 1 ml of 10 % formaldehyde in PBS (1/10 diluted Formalin with PBS) over night and stained using 0.5 % crystal violet (0.5 g of powder form Crystal Violet in 100 mL of 99.5 % Ethanol) and after 1 h they were washed gently with tap water and left to dry at room temperature.

For the CHIKV positive abdomens, the corresponding legs and heads were homogenized and assayed as described to determine the dissemination status of the mosquitoes. Detection of the virus in the mosquito abdomen and not its legs or head was an indication that the mosquito had a non-disseminated infection (limited to its midgut), while detection of the virus in the legs and/or head was an indication of a disseminated infection [56-58].

Ethical considerations

KEMRI Animal Care and Use Committee (ACUC), Scientific Steering Committee (SSC) and Ethical Review Committee (ERC) approved all the procedures used in this study. The guidelines were strictly adhered to during the research.

Statistical analyses

The collected data were analyzed using SPSS version 20.0 software package. Differences in mosquito counts due to differences in treatments were analyzed using chi-square test. A logit model was utilized in showing the nominal main effects of the region, temperature, EIP and their interactions on the infection rates of CHIKV. MIR was computed as (Number of positive midgut infections divided by Total number of mosquitoes tested) × 100 % [59, 60] for day 7, 10 and 13 in the order. We defined the midgut infection rate as the percentage of mosquitoes tested that contained the virus in their abdomen/midgut and the dissemination rates in the legs and heads as the percentage of infected (abdomen/midgut positive) mosquitoes that contained virus in their legs and/or heads respectively. The differences between these groups were deemed statistically significant at p < 0.05.

Results

Midgut infection rates for Coastal and Western Kenya Ae. aegypti population

The 7, 10 and 13 days post infection (DPI) midgut infection rates of Ae. aegypti population for CHIKV in Coastal and Western Kenya Ae. aegypti reared at EIT of 26 and 32 °C is shown in Table 1 with temporal midgut infection rate vis-a-vis temperature ranges trends shown in Fig. 1. A binary logistic regression model showing the nominal effects of the region, EIT, DPI and their interactions on the Midgut infection rates (MIR) for CHIKV is presented in Table 2 (Model summary: −2 Log likelihood = 458.345, Nagelkerke R2 = 0.5323). Regardless of the EIT, the MIR of Ae. aegypti sampled from Coastal Region was significantly (p < 0.05) higher than those sampled from Western Kenya, with no statistical differences observed for the coastal Ae. aegypti at EIT 26 and at 32 °C. Meanwhile the MIR of Ae. aegypti from the Western Kenya Region (26 °C) was significantly (p < 0.05) affected by the EIT, with mosquito reared at EIT 32 °C exhibiting higher MIR than those reared at EIT 26 °C. The 2 way interactions between region, EIT and DPI significantly affected the MIR (p < 0.05). We also established a significant (p < 0.05) interactive effects of the region, EIT and DPI on MIR.

Table 1

Midgut infection rates of 7, 10 and 13 DPI of CHIKV in Coastal and Western Kenya Ae. aegypti reared at EIT of 26 and 32 °C

Region	Number midgut tested (n)	EITa	DPIb	Number of midgut infections	MIRc (%)
Coastal	156	26 °C	7		17.9
			10		23.1
			13		26.9
	173	32 °C	7		17.3
			10		26.0
			13		16.2
Western Kenya	146	26 °C	7		5.5
			10		4.1
			13		13.7
	156	32 °C	7		22.4
			10		16.7
			13		16.7

Within each population and temperature conditions, the number of tested mosquitoes is the same for all the days of sampling

aEIT = Extrinsic Incubation Temperature

bDPI = Days Post Infection

cMIR = Midgut Infection Rate

Fig. 1

Proportion ± 95 % confidence interval of Coastal (a) and Western Kenya (b) Ae. aegypti infected at day 7, 10 and 13 post-infection at temperature levels of 26 and 32 °C

Table 2

Logit model of the nominal main effects of the region, EIT and DPI and their interactions on the midgut infection rates of CHIKV

Effects	E	Std Error	df	Wald	Sig	Chi-square	EXP(B)
Intercept	−0.742	4.7348	1	6.8119	0.0037	5.9431	0.476
Region	1.5207	5.2578	1	10.1012	0.0000	37.6654	4.5754
EIT	1.039	3.6764	1	−6.3544	0.0021	17.8042	2.827
DPI	−0.602	4.3498	1	7.3677	0.0027	6.1545	0.548
Region*EIT	0.459	3.1241	2	8.4674	0.0004	9.1973	1.5825
Region*DPI	1.518	9.3498	2	−19.2382	0.0000	31.4455	4.563
EIT *DPI	0.690	6.0174	2	7.01305	0.0002	21.1073	1.994
Region* EIT *DPI	−0.6027	2.1132	3	0.0065	0.0331	4.3053	0.5473

EIT = Extrinsic Incubation Temperature, DPI = Days Post Infection

Dissemination rates of CHIKV in Coastal and Western Kenya Ae. aegypti population

The dissemination rates of infected Ae. aegypti for CHIKV from the Coastal Region and Western Kenya at 7, 10 and 13 DPI and at EIT of 26 and 32 °C is shown in Table 3. The disseminated infection rates for the CHIKV in Ae. aegypti in the legs (DIR-L) was higher in mosquito sampled from higher ambient temperature setting (Coastal Region) regardless of the EIT while those from ambient temperature of 26 °C (Western Kenya) dissemination rates was significantly higher at higher EIT of 32 °C. There were no significant interactions (p > 0.05) between region and EIT on the dissemination of CHIKV from the midgut to the head, indicating that the trends in the infection rates by CHIKV remained similar at the coastal and Western region regardless of the EIT. Notably, there were no significant (p > 0.05) interaction between region, EIT and DPI on DIR on the legs and head.

Table 3

Dissemination rates of CHIKV to the legs and head in Coastal and Western Kenya Ae. aegypti reared at Extrinsic Incubation Temperature of 26 and 32 °C

				Number infected(b)		Dissemination rates (%) (c)
Region	Number of midgut infections (a)	EIT	DPI	Legs	Head	Legs	Head
Coastal Region	106	26 °C	7	19	11	17.9	10.4
			10	27	3	25.5	2.8
			13	18	3	17.0	2.8
	103	32 °C	7	7	3	6.8	2.9
			10	21	17	20.4	16.5
			13	30	27	29.1	26.2
Western Kenya	34	26 °C	7	9	9	26.5	26.5
			10	4	6	11.8	17.6
			13	7	7	20.6	20.6
	87	32 °C	7	25	22	28.7	25.3
			10	15	7	17.2	8.0
			13	23	20	26.4	23.0

Dissemination rate (c) for legs or head in that order is provided as [(b dived by a) × 100 %] where numerator b corresponds to number of infected legs or head respectively

Discussion

Arboviruses are ecologically complex, and interactions between larval mosquitoes and their aquatic environment can influence adult transmission dynamics. Moreover, due to the impact of climate on vector ecology, competence and their risk of transmitting viruses may be sensitive to projected changes in global temperatures. In this study, we evaluated the effect of ambient temperatures and changes of EIT on the risk of vector transmission and competence of the Ae. aegypti for CHIKV. We provide evidence that the incubation temperatures of vector directly impact virus transmission by influencing the likelihood of infection and dissemination of CHIKV. We established that the MIR of Ae. aegypti sampled from the coastal area with ambient temperature of 32 °C was higher than those sampled from the western Kenya that has ambient temperature 26 °C regardless of the EIT. Meanwhile for Ae. aegypti emanating from lower ambient temperature of 26 °C, there was increased MIR when EIT was increased from 26 to 32 °C. This suggests that virus transmission is likely to be affected more by higher environmental temperature due to possible effects of the temperature on the biological processes moderating the vector competence [61]. It has earlier been noted that temperature may limit virus transmission in areas where the vectors is present noting that an increase in environmental temperature for adult mosquitoes reduces the EIP most likely due to an increase in the metabolism of the adult mosquito and replication speed of the virus [28, 61, 62]. Equally, temperature changes experienced in the immature stages of the mosquito development before infection may affect vector virus interactions by changing physical and physiological characteristics of mid-gut barriers which would impact virus infection and transmission [63, 64]. This is in agreement with previous studies which have established that ambient temperature affect the biological processes of mosquitoes and plays a key role in modulating mosquito vector competence for pathogens [65-67]. Previous studies have indicated that increases in adult-holding incubation temperatures have usually been associated with enhanced vector competence [62, 68–74]. However, some studies have identified reduced vector competence and activity in nature associated with increases in incubation temperature [64, 75–77]. It has long been recognized that increases in incubation temperature reduce the extrinsic incubation period (the time from initial acquisition of pathogens until transmission is possible) [28], which render virus transmission more likely under such incubation period. Along the same lines, increases in temperature reduce the adult lifespan of mosquitoes and may impinge transmission [69, 70, 78]. Temperature effects may drastically alter risk of disease transmission, especially under conditions where the extrinsic incubation period approaches the lifespan of the mosquito. This result differs with other systems where arboviral vector competence was reduced in female mosquitoes that were reared at higher compared to lower temperatures [68–70, 78]. However, vector capacity of a mosquito population is a complex phenomenon that is influenced by a number of factors such as host seeking behavior and longevity of the infected mosquitoes apart from temperature and inherent factors [79] and thus further studies are recommended on how these factors can combine to affect the MIR.

Mosquito susceptibility to arbovirus infection resides primarily in the midgut and can vary greatly between mosquito species and geographical strains of the same species and even within individuals of the same population [80]. Vector competence, which is the capacity of an arthropod to acquire an infection and transmit it to a subsequent host, can greatly vary among individuals and between populations [56] and has been previously linked to genetics [81] as well as by climate variables such as temperature [70, 82]. Disseminated infection is generally accepted as a measure of a mosquito’s ability to transmit a virus through biting [56, 70]. The rate of dissemination, when expressed as a percentage of the number of mosquitoes infected, may provide information about the effect of a “midgut escape barrier” moderating whether gut infections are able to disseminate into the hemolymph. In the current study, the dissemination rates of infected Ae. aegypti for CHIKV in the legs was high at higher ambient temperature regardless of the EIT. Notably, the disseminated infection rates for the CHKV in Ae. aegypti in the legs was higher in mosquito emanating from ambient temperature 32 °C (Coastal Region) regardless of the EIT while those from ambient temperature of 26 °C (Western Kenya) dissemination rates was significantly higher at higher EIT of 32 °C. These results suggest that the midgut barriers preventing dissemination were strongly influenced by the ambient and rearing temperature. Thus, it can be speculated that there may be an increased midgut escape barrier in mosquitoes derived from the higher rearing temperatures. At temperature of 26 °C during the adult stage resulted in the lowest rates of viral dissemination. Rates of dissemination were higher at 32 °C relative to cooler holding temperatures of adults. These results corroborate observations found for laboratory studies examining susceptibility to dengue virus infection and length of the extrinsic incubation period in Ae. albopictus and Ae. aegypti [83-85]. However, we found no association between vector dissemination in between the midgut and the head. The explanation for these observed effects of mosquitoes with disseminated infections is not entirely clear, but it does suggest complex effects of temperature on virus infection and dissemination and by extension, mosquito competence.

Conclusion

The current study underscores the importance of the environmental and incubation temperature in dictating the vector epidemiological risk of the virus in the human populations. Vector competence was higher in mosquito populations reared under high temperatures which weakens the midgut infection barrier. Hence, suggesting Lamu population is more susceptible to CHIKV therefore having a weaker mid gut infection barrier than the Trans Nzoia population. This study further demonstrates the importance of examining the course of infection at various ambient temperatures and EIT between the two mosquito populations. Vector control measures should be triggered as an integral component of climate change policies discourse to prevent un-anticipated transmission transition of such vector ecology dependent infectious pathogens. Although our results show differences in vector competence, other factors (mosquito densities, feeding behavior, mosquito survival rates) composing the vector capacity, are needed to assess more accurately the risk of CHIKV transmission alongside virus titers in the context of dissemination. Future studies should explore the connection between larval rearing temperature-infection patterns observed in the laboratory and patterns in the field, and how climate and climate change related factors may continue to impact the mosquito larval environment and the epidemiology of CHIKV.