Literature DB >> 27347495

Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts.

Joseph C Chen1, Nadia R Roan2.   

Abstract

Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.

Entities:  

Year:  2015        PMID： 27347495      PMCID： PMC4918807          DOI： 10.21769/bioprotoc.1623

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  7 in total

1.  Perivascular human endometrial mesenchymal stem cells express pathways relevant to self-renewal, lineage specification, and functional phenotype.

Authors:  Trimble L B Spitzer; Angela Rojas; Zara Zelenko; Lusine Aghajanova; David W Erikson; Fatima Barragan; Michelle Meyer; John S Tamaresis; Amy E Hamilton; Juan C Irwin; Linda C Giudice
Journal:  Biol Reprod       Date:  2012-02-29       Impact factor: 4.285

2.  Stromal-epithelial interactions modulate estrogen responsiveness in normal human endometrium.

Authors:  E Pierro; F Minici; O Alesiani; F Miceli; C Proto; I Screpanti; S Mancuso; A Lanzone
Journal:  Biol Reprod       Date:  2001-03       Impact factor: 4.285

3.  Normal human endometrium in cell culture. I. Separation and characterization of epithelial and stromal components in vitro.

Authors:  D Kirk; R J King; J Heyes; L Peachey; P J Hirsch; R W Taylor
Journal:  In Vitro       Date:  1978-08

4.  Normal human endometrium in cell culture.

Authors:  D Kirk; J C Irwin
Journal:  Methods Cell Biol       Date:  1980       Impact factor: 1.441

5.  Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts.

Authors:  Joseph C Chen; Brittni A Johnson; David W Erikson; Terhi T Piltonen; Fatima Barragan; Simon Chu; Nargis Kohgadai; Juan C Irwin; Warner C Greene; Linda C Giudice; Nadia R Roan
Journal:  Hum Reprod       Date:  2014-03-13       Impact factor: 6.918

6.  Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production.

Authors:  Joseph C Chen; David W Erikson; Terhi T Piltonen; Michelle R Meyer; Fatima Barragan; Ramsey H McIntire; John S Tamaresis; Kim Chi Vo; Linda C Giudice; Juan C Irwin
Journal:  Fertil Steril       Date:  2013-07-10       Impact factor: 7.329

7.  The isolation and long-term culture of normal human endometrial epithelium and stroma. Expression of mRNAs for angiogenic polypeptides basally and on oestrogen and progesterone challenges.

Authors:  L Zhang; M C Rees; R Bicknell
Journal:  J Cell Sci       Date:  1995-01       Impact factor: 5.285
  7 in total
  12 in total

1.  Has the time come to include low-level laser photobiomodulation as an adjuvant therapy in the treatment of impaired endometrial receptivity?

Authors:  Doaa Abdelmageed El Faham; Mohamed Amr Hussein Elnoury; Mona Ibraheim Morsy; Marwa Abdelmoneim El Shaer; Ghada Mohammed Nour Eldin; Osama Mahmoud Azmy
Journal:  Lasers Med Sci       Date:  2018-03-15       Impact factor: 3.161

2.  Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma.

Authors:  Jeremy W Martin; Joseph C Chen; Jason Neidleman; Keiji Tatsumi; James Hu; Linda C Giudice; Warner C Greene; Nadia R Roan
Journal:  Biol Reprod       Date:  2018-08-01       Impact factor: 4.285

3.  Seminal Plasma-Derived Extracellular-Vesicle Fractions from HIV-Infected Men Exhibit Unique MicroRNA Signatures and Induce a Proinflammatory Response in Cells Isolated from the Female Reproductive Tract.

Authors:  Erika G Marques de Menezes; Karen Jang; Ashley F George; Mette Nyegaard; Jason Neidleman; Heather C Inglis; Ali Danesh; Xutao Deng; Amirali Afshari; Young H Kim; Jean-Noël Billaud; Kara Marson; Christopher D Pilcher; Satish K Pillai; Philip J Norris; Nadia R Roan
Journal:  J Virol       Date:  2020-07-30       Impact factor: 5.103

4.  Niche matters: The comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus.

Authors:  Masuma Khatun; Anna Sorjamaa; Marika Kangasniemi; Meeri Sutinen; Tuula Salo; Annikki Liakka; Petri Lehenkari; Juha S Tapanainen; Olli Vuolteenaho; Joseph C Chen; Siri Lehtonen; Terhi T Piltonen
Journal:  PLoS One       Date:  2017-04-18       Impact factor: 3.240

5.  Semen inhibits Zika virus infection of cells and tissues from the anogenital region.

Authors:  Janis A Müller; Mirja Harms; Franziska Krüger; Rüdiger Groß; Simone Joas; Manuel Hayn; Andrea N Dietz; Sina Lippold; Jens von Einem; Axel Schubert; Manuela Michel; Benjamin Mayer; Mirko Cortese; Karen S Jang; Nathallie Sandi-Monroy; Miriam Deniz; Florian Ebner; Olli Vapalahti; Markus Otto; Ralf Bartenschlager; Jean-Philippe Herbeuval; Jonas Schmidt-Chanasit; Nadia R Roan; Jan Münch
Journal:  Nat Commun       Date:  2018-06-07       Impact factor: 14.919

6.  Functional expression of the mechanosensitive PIEZO1 channel in primary endometrial epithelial cells and endometrial organoids.

Authors:  Aurélie Hennes; Katharina Held; Matteo Boretto; Katrien De Clercq; Charlotte Van den Eynde; Arne Vanhie; Nele Van Ranst; Melissa Benoit; Catherine Luyten; Karen Peeraer; Carla Tomassetti; Christel Meuleman; Thomas Voets; Hugo Vankelecom; Joris Vriens
Journal:  Sci Rep       Date:  2019-02-11       Impact factor: 4.379

7.  Effects of the levonorgestrel-containing intrauterine device, copper intrauterine device, and levonorgestrel-containing oral contraceptive on susceptibility of immune cells from cervix, endometrium and blood to HIV-1 fusion measured ex vivo.

Authors:  Marielle Cavrois; Joan F Hilton; Nadia R Roan; Margaret Takeda; Dominika Seidman; Sarah Averbach; Eric Chang; Nandhini Raman; Ruth Greenblatt; Barbara L Shacklett; Karen Smith-McCune
Journal:  PLoS One       Date:  2019-08-22       Impact factor: 3.240

Review 8.  Organoid systems to study the human female reproductive tract and pregnancy.

Authors:  Lama Alzamil; Konstantina Nikolakopoulou; Margherita Y Turco
Journal:  Cell Death Differ       Date:  2020-06-03       Impact factor: 15.828

9.  Seminal plasma promotes decidualization of endometrial stromal fibroblasts in vitro from women with and without inflammatory disorders in a manner dependent on interleukin-11 signaling.

Authors:  Ashley F George; Karen S Jang; Mette Nyegaard; Jason Neidleman; Trimble L Spitzer; Guorui Xie; Joseph C Chen; Eytan Herzig; Anders Laustsen; Erika G Marques de Menezes; Sahar Houshdaran; Christopher D Pilcher; Philip J Norris; Martin R Jakobsen; Warner C Greene; Linda C Giudice; Nadia R Roan
Journal:  Hum Reprod       Date:  2020-03-27       Impact factor: 6.918

Review 10.  Building a stem cell-based primate uterus.

Authors:  Sophie Bergmann; Magdalena Schindler; Clara Munger; Christopher A Penfold; Thorsten E Boroviak
Journal:  Commun Biol       Date:  2021-06-17
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