| Literature DB >> 27345973 |
Tong Li1, Carlota Pires1, Troels T Nielsen2, Gunhild Waldemar2, Lena E Hjermind2, Jørgen E Nielsen2, Andras Dinnyes3, Poul Hyttel1, Kristine K Freude4.
Abstract
Skin fibroblasts were obtained from a 48-year-old presymptomatic woman carrying a A79V mutation in the presenilin 1 gene (PSEN1), causing Alzheimer's disease (AD). Induced pluripotent stem cell (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. A79V-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported A79V-iPSCs line may be a useful resource for in vitro modeling of familial AD.Entities:
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Year: 2016 PMID： 27345973 DOI： 10.1016/j.scr.2016.01.002
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020