Priscila Vieira da Silva1, Jéssica Antonini Troiano2, Ana Cláudia M S Nakamune2, Juliano Pelim Pessan1, Cristina Antoniali3. 1. Graduate Program in Dental Science, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil; Department of Pediatric Dentistry and Public Heatlh, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil. 2. Multicenter Graduate Program in Physiological Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil; Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil. 3. Graduate Program in Dental Science, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil; Multicenter Graduate Program in Physiological Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil; Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil. Electronic address: crisant@foa.unesp.br.
Abstract
OBJECTIVE: This study aimed to evaluate the oxidative stress levels and the enzymatic and non-enzymatic antioxidant systems in saliva of toddlers with severe early childhood caries (S-ECC). DESIGN: Unstimulated saliva samples were collected at the morning from 0 to 3 year-old S-ECC (n=30) or caries-free (CF) children (n=30/group) for evaluation of oxidative stress (OS) and total antioxidant capacity (TAC), which were measured by the ferric reducing antioxidant power (FRAP) assay, as well as to assess the activity of enzymatic (superoxide dismutase, SOD) and non-enzymatic (uric acid, UA) antioxidant systems, respectively. Data were analyzed by Student's t-test (p<0.05). RESULTS: Significantly higher protein levels were observed in saliva of S-ECC children (0.083mg/mL) than in the CF group (0.070mg/mL). Oxidative damage was significantly lower in saliva of S-ECC children (0.0019μmol/L/mg protein) than in CF children (0.0039μmol/L/mg protein), while salivary TAC (61.5μmol/L), SOD activity (36.6 UE/mL) and uric acid (7.05mg/mL) were significantly higher in saliva of S-ECC when compared to the CF group (49.1μmol/L, 26.8 UE/mL and 5.02mg/mL, respectively for TAC, SOD and UA). CONCLUSION: Oxidative stress levels were significantly lower in saliva of S-ECC children, what might be associated with the increased activity of salivary enzymatic (SOD) and non-enzymatic (uric acid) antioxidant systems.
OBJECTIVE: This study aimed to evaluate the oxidative stress levels and the enzymatic and non-enzymatic antioxidant systems in saliva of toddlers with severe early childhood caries (S-ECC). DESIGN: Unstimulated saliva samples were collected at the morning from 0 to 3 year-old S-ECC (n=30) or caries-free (CF) children (n=30/group) for evaluation of oxidative stress (OS) and total antioxidant capacity (TAC), which were measured by the ferric reducing antioxidant power (FRAP) assay, as well as to assess the activity of enzymatic (superoxide dismutase, SOD) and non-enzymatic (uric acid, UA) antioxidant systems, respectively. Data were analyzed by Student's t-test (p<0.05). RESULTS: Significantly higher protein levels were observed in saliva of S-ECC children (0.083mg/mL) than in the CF group (0.070mg/mL). Oxidative damage was significantly lower in saliva of S-ECC children (0.0019μmol/L/mg protein) than in CF children (0.0039μmol/L/mg protein), while salivary TAC (61.5μmol/L), SOD activity (36.6 UE/mL) and uric acid (7.05mg/mL) were significantly higher in saliva of S-ECC when compared to the CF group (49.1μmol/L, 26.8 UE/mL and 5.02mg/mL, respectively for TAC, SOD and UA). CONCLUSION: Oxidative stress levels were significantly lower in saliva of S-ECC children, what might be associated with the increased activity of salivary enzymatic (SOD) and non-enzymatic (uric acid) antioxidant systems.
Authors: Mária Džunková; Daniel Martinez-Martinez; Roman Gardlík; Michal Behuliak; Katarína Janšáková; Nuria Jiménez; Jorge F Vázquez-Castellanos; Jose Manuel Martí; Giuseppe D'Auria; H M H N Bandara; Amparo Latorre; Peter Celec; Andrés Moya Journal: NPJ Biofilms Microbiomes Date: 2018-11-27 Impact factor: 7.290
Authors: Helena C Barroso; Murilo E Graton; Simone R Potje; Jéssica A Troiano; Lilian X Silva; Ana Cláudia M S Nakamune; Cristina Antoniali Journal: Data Brief Date: 2022-02-03