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Literature DB >> 27327211

Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.

Wesley Cardoso Generoso¹, Manuela Gottardi², Mislav Oreb³, Eckhard Boles⁴.

Abstract

CRISPR-Cas has become a powerful technique for genetic engineering of yeast. Here, we present an improved version by using only one single plasmid expressing Cas9 and one or two guide-RNAs. A high gene deletion efficiency was achieved even with simultaneous recombination cloning of the plasmid and deletion in industrial strains.

Entities: Species

Keywords: CRISPR-Cas9; Metabolic engineering; Sacchromyces cerevisiae

Mesh：

Year: 2016 PMID： 27327211 DOI： 10.1016/j.mimet.2016.06.020

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

44 in total

Review 1. CRISPR-Cas9: From a bacterial immune system to genome-edited human cells in clinical trials.

Authors: Leonhard Kick; Marion Kirchner; Sabine Schneider
Journal: Bioengineered Date: 2017-03-13 Impact factor: 3.269

2. The gal80 Deletion by CRISPR-Cas9 in Engineered Saccharomyces cerevisiae Produces Artemisinic Acid Without Galactose Induction.

Authors: Limei Ai; Weiwei Guo; Wei Chen; Yun Teng; Liping Bai
Journal: Curr Microbiol Date: 2019-08-07 Impact factor: 2.188

3. The unstructured linker of Mlh1 contains a motif required for endonuclease function which is mutated in cancers.

Authors: Kendall A Torres; Felipe A Calil; Ann L Zhou; Matthew L DuPrie; Christopher D Putnam; Richard D Kolodner
Journal: Proc Natl Acad Sci U S A Date: 2022-10-10 Impact factor: 12.779

Review 4. Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing in Saccharomyces cerevisiae.

Authors: Jacob S Antony; John M Hinz; John J Wyrick
Journal: Front Bioeng Biotechnol Date: 2022-05-30

5. Partners in crime: Tbf1 and Vid22 promote expansions of long human telomeric repeats at an interstitial chromosome position in yeast.

Authors: Elina A Radchenko; Anna Y Aksenova; Kirill V Volkov; Alexander A Shishkin; Youri I Pavlov; Sergei M Mirkin
Journal: PNAS Nexus Date: 2022-06-08

10. Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression.

Authors: Virginia Schadeweg; Eckhard Boles
Journal: Biotechnol Biofuels Date: 2016-11-25 Impact factor: 6.040

Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.

Review 1. CRISPR-Cas9: From a bacterial immune system to genome-edited human cells in clinical trials.

2. The gal80 Deletion by CRISPR-Cas9 in Engineered Saccharomyces cerevisiae Produces Artemisinic Acid Without Galactose Induction.

3. The unstructured linker of Mlh1 contains a motif required for endonuclease function which is mutated in cancers.

Review 4. Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing in Saccharomyces cerevisiae.

5. Partners in crime: Tbf1 and Vid22 promote expansions of long human telomeric repeats at an interstitial chromosome position in yeast.

6. eGFP Gene Integration in HO: A Metabolomic Impact?

7. The HOPS tethering complex is required to maintain signaling endosome identity and TORC1 activity.

8. gEL DNA: A Cloning- and Polymerase Chain Reaction-Free Method for CRISPR-Based Multiplexed Genome Editing.

9. Defining intermediates and redundancies in coenzyme Q precursor biosynthesis.

10. Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression.