Literature DB >> 27325258

Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells.

Anika Nagelkerke1, Paul N Span2.   

Abstract

Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against γ-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.

Entities:  

Keywords:  DNA damage repair; Genomic instability; Histone 2A; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Telomere dysfunction

Mesh：

Substances：

Year:  2016        PMID： 27325258     DOI： 10.1007/978-3-319-26666-4_1

Source DB:  PubMed          Journal:  Adv Exp Med Biol        ISSN： 0065-2598            Impact factor:   2.622


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