Maria Jesus Alvarez-Cubero1, Manrique Pascual-Geler2, Luis Javier Martinez-Gonzalez3, Manuela Expósito Ruiz4, Maria Saiz5, Jose Manuel Cozar2, Jose Antonio Lorente6. 1. Liquid biopsy and metastasis research group and Genomic Unit, GENyO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain. Electronic address: mjesusac@ugr.es. 2. Service of Urology, University Hospital Virgen de las Nieves, Granada, Spain. 3. Liquid biopsy and metastasis research group and Genomic Unit, GENyO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain. 4. Fundación para la Investigación Biosanitaria de Andalucía Oriental-Alejandro Otero (FIBAO), Hospital Virgen de las Nieves, Granada, Spain. 5. Laboratory of Genetic Identification, Legal Medicine and Toxicology Department, Faculty of Medicine, University of Granada, Granada, Spain. 6. Liquid biopsy and metastasis research group and Genomic Unit, GENyO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain; Laboratory of Genetic Identification, Legal Medicine and Toxicology Department, Faculty of Medicine, University of Granada, Granada, Spain.
Abstract
BACKGROUND: There is contradictory evidence of the effects that environmental factors-dietary habits (ingestion rates of red meat, soy products, fish, etc.) and work environment (exposure to metals, pesticides, several toxic products, etc.)-and KLK3, AR, RNASEL, MSR1, and ELAC2 expression patterns have on prostate cancer (PCa). In our study, we investigated the potential association between KLK3, AR, RNASEL, MSR1, and ELAC2 polymorphisms, expression patterns, exposure to environmental factors, and PCa in a Spanish cohort. Blood and fresh tissue samples were collected from 322 subjects with prostate-specific antigen (PSA)>4ng/ml to determine their genotypes (RNASEL, MSR1, and ELAC2) and assess messenger ribonucleic acid expression levels (by quantitative amplification testing). MAIN FINDINGS: Among clinical parameters, a 63.6% of patients with CC variants in rs11545302 (ELAC2) had PSA>20ng/ml (P = 0.008), and rs486907 (RNASEL), with 52.8% of patients with CT variants with Gleason score>7. Regarding TNM stage, patients with GG variants, rs4792311 (ELAC2) generally had stage 1 tumors. Genetic expression analysis revealed RNASEL (P = 0.007) was underexpressed in PCa tissue, whereas KLK3 (P = 0.041) was overexpressed. As to environmental factors, the intake of dried fruits (P = 0.036) and practice of sports (P = 0.024) revealed an effect in PCa. Moreover, environmental factors were observed to affect gene expression patterns. Thus, RNASEL (P = 0.018) and ELAC2 (P = 0.023) were found to be underexpressed in patients who ate processed foods frequently; MSR1 (P = 0.024) and AR (P = 0.004) were underexpressed in patients who did not practice sports; and KLK3 (P = 0.039; P = 0.046) underexpressed in patients exposed to dust and toxic products. CONCLUSIONS: This is the first study to analyze the correlation between RNASEL, MSR1, and ELAC2 genotypes and messenger ribonucleic acid expression in PCa. RNASEL and KLK3 show different expression patterns in normal vs. tumor tissue, which supports their reported relevance in human cancer. The results obtained confirm that RNASEL plays a crucial role in PCa. Environmental factors such as exercise, exposure to toxic agents, and intake of processed foods are associated with PCa.
BACKGROUND: There is contradictory evidence of the effects that environmental factors-dietary habits (ingestion rates of red meat, soy products, fish, etc.) and work environment (exposure to metals, pesticides, several toxic products, etc.)-and KLK3, AR, RNASEL, MSR1, and ELAC2 expression patterns have on prostate cancer (PCa). In our study, we investigated the potential association between KLK3, AR, RNASEL, MSR1, and ELAC2 polymorphisms, expression patterns, exposure to environmental factors, and PCa in a Spanish cohort. Blood and fresh tissue samples were collected from 322 subjects with prostate-specific antigen (PSA)>4ng/ml to determine their genotypes (RNASEL, MSR1, and ELAC2) and assess messenger ribonucleic acid expression levels (by quantitative amplification testing). MAIN FINDINGS: Among clinical parameters, a 63.6% of patients with CC variants in rs11545302 (ELAC2) had PSA>20ng/ml (P = 0.008), and rs486907 (RNASEL), with 52.8% of patients with CT variants with Gleason score>7. Regarding TNM stage, patients with GG variants, rs4792311 (ELAC2) generally had stage 1 tumors. Genetic expression analysis revealed RNASEL (P = 0.007) was underexpressed in PCa tissue, whereas KLK3 (P = 0.041) was overexpressed. As to environmental factors, the intake of dried fruits (P = 0.036) and practice of sports (P = 0.024) revealed an effect in PCa. Moreover, environmental factors were observed to affect gene expression patterns. Thus, RNASEL (P = 0.018) and ELAC2 (P = 0.023) were found to be underexpressed in patients who ate processed foods frequently; MSR1 (P = 0.024) and AR (P = 0.004) were underexpressed in patients who did not practice sports; and KLK3 (P = 0.039; P = 0.046) underexpressed in patients exposed to dust and toxic products. CONCLUSIONS: This is the first study to analyze the correlation between RNASEL, MSR1, and ELAC2 genotypes and messenger ribonucleic acid expression in PCa. RNASEL and KLK3 show different expression patterns in normal vs. tumor tissue, which supports their reported relevance in humancancer. The results obtained confirm that RNASEL plays a crucial role in PCa. Environmental factors such as exercise, exposure to toxic agents, and intake of processed foods are associated with PCa.
Authors: Chen Jin; Rui Li; Tuo Deng; Zixia Lin; Haoqi Li; Yan Yang; Qing Su; Jingxian Wang; Yi Yang; Juejin Wang; Gang Chen; Yi Wang Journal: Front Nutr Date: 2022-07-18
Authors: Inmaculada Robles-Fernandez; Luis Javier Martinez-Gonzalez; Manrique Pascual-Geler; Jose Manuel Cozar; Ignacio Puche-Sanz; Maria Jose Serrano; Jose Antonio Lorente; Maria Jesus Alvarez-Cubero Journal: PLoS One Date: 2017-10-05 Impact factor: 3.240
Authors: Ahmad M Alamri; Xuefeng Liu; Jan K Blancato; Bassem R Haddad; Weisheng Wang; Xiaogang Zhong; Sujata Choudhary; Ewa Krawczyk; Bhaskar V Kallakury; Bruce J Davidson; Priscilla A Furth Journal: Dis Model Mech Date: 2018-01-29 Impact factor: 5.758