Hossein Faramarzi1, Davood Mehrabani2, Maryam Fard3, Maryam Akhavan3, Sona Zare4, Shabnam Bakhshalizadeh5, Amir Manafi6, Somaieh Kazemnejad7, Reza Shirazi8. 1. Department of Infectious Diseases, Larestan School of Medical Sciensce, Larestan, Iran; 2. Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran;; Department of Regenerative Medicine, University of Manitoba, Winnipeg, MB, Canada; 3. Department of Anatomical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran; 4. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran; 5. Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; 6. Department of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 7. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; 8. Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Menstrual blood-derived stem cells (MenSCs) are a novel source of stem cells that can be easily isolated non-invasively from female volunteered donor without ethical consideration. These mesenchymal-like stem cells have high rate of proliferation and possess multi lineage differentiation potency. This study was undertaken to isolate the MenSCs and assess their potential in differentiation into epidermal lineage. METHODS: About 5-10 ml of menstrual blood (MB) was collected using sterile Diva cups inserted into vagina during menstruation from volunteered healthy fertile women aged between 22-30 years. MB was transferred into Falcon tubes containing phosphate buffered saline (PBS) without Ca2(+) or Mg2(+) supplemented with 2.5 µg/ml fungizone, 100 µg/mL streptomycin, 100 U/mL penicillin and 0.5 mM EDTA. Mononuclear cells were separated using Ficoll-Hypaque density gradient centrifugation and washed out in PBS. The cell pellet was suspended in DMEM-F12 medium supplemented with 10% FBS and cultured in tissue culture plates. The isolated cells were co-cultured with keratinocytes derived from the foreskin of healthy newborn male aged 2-10 months who was a candidate for circumcision for differentiation into epidermal lineage. RESULTS: The isolated MenSCs were adhered to the plate and exhibited spindle-shaped morphology. Flow cytometric analysis revealed the expression of mesenchymal markers of CD10, CD29, CD73, and CD105 and lack of hematopoietic stem cells markers. An early success in derivation of epidermal lineage from MenSCs was visible. CONCLUSION: The MenSCs are a real source to design differentiation to epidermal cells that can be used non-invasively in various dermatological lesions and diseases.
BACKGROUND: Menstrual blood-derived stem cells (MenSCs) are a novel source of stem cells that can be easily isolated non-invasively from female volunteered donor without ethical consideration. These mesenchymal-like stem cells have high rate of proliferation and possess multi lineage differentiation potency. This study was undertaken to isolate the MenSCs and assess their potential in differentiation into epidermal lineage. METHODS: About 5-10 ml of menstrual blood (MB) was collected using sterile Diva cups inserted into vagina during menstruation from volunteered healthy fertile women aged between 22-30 years. MB was transferred into Falcon tubes containing phosphate buffered saline (PBS) without Ca2(+) or Mg2(+) supplemented with 2.5 µg/ml fungizone, 100 µg/mL streptomycin, 100 U/mL penicillin and 0.5 mM EDTA. Mononuclear cells were separated using Ficoll-Hypaque density gradient centrifugation and washed out in PBS. The cell pellet was suspended in DMEM-F12 medium supplemented with 10% FBS and cultured in tissue culture plates. The isolated cells were co-cultured with keratinocytes derived from the foreskin of healthy newborn male aged 2-10 months who was a candidate for circumcision for differentiation into epidermal lineage. RESULTS: The isolated MenSCs were adhered to the plate and exhibited spindle-shaped morphology. Flow cytometric analysis revealed the expression of mesenchymal markers of CD10, CD29, CD73, and CD105 and lack of hematopoietic stem cells markers. An early success in derivation of epidermal lineage from MenSCs was visible. CONCLUSION: The MenSCs are a real source to design differentiation to epidermal cells that can be used non-invasively in various dermatological lesions and diseases.
Authors: Clara V Alvarez; Montserrat Garcia-Lavandeira; Maria E R Garcia-Rendueles; Esther Diaz-Rodriguez; Angela R Garcia-Rendueles; Sihara Perez-Romero; Tania Vila Vila; Joana S Rodrigues; Pamela V Lear; Susana B Bravo Journal: J Mol Endocrinol Date: 2012-08-30 Impact factor: 5.098
Authors: Jimena Cuenca; Alice Le-Gatt; Valentina Castillo; Jose Belletti; Macarena Díaz; Mónica Kurte G; Paz L Gonzalez; Francisca Alcayaga-Miranda; Christina M A P Schuh; Fernando Ezquer; Marcelo Ezquer; Maroun Khoury Journal: Front Physiol Date: 2018-05-14 Impact factor: 4.566