Background: In recent years, researchers discovered that menstrual blood-derived stem cells (MenSCs) have the potential to differentiate into a wide range of tissues including the chondrogenic lineage. In this study, we aimed to investigate the effect of MenSCs encapsulated in fibrin glue (FG) on healing of osteochondral defect in rabbit model. Methods: We examined the effectiveness of MenSCs encapsulated in FG in comparison with FG alone in the repair of osteochondral defect (OCD) lesions of rabbit knees after 12 and 24 weeks. Results: Macroscopical evaluation revealed that the effectiveness of MenSCs incorporation with FG is much higher than FG alone in repair of OCD defects. Indeed, histopathological evaluation of FG + MenSCs group at 12 weeks post-transplantation demonstrated that defects were filled with hyaline cartilage-like tissue with proper integration, high content of glycosaminoglycan and the existence of collagen fibers especially collagen type II, as well as by passing time (24 weeks post-transplantation), the most regenerated tissue in FG + MenSCs group was similar to hyaline cartilage with relatively good infill and integration. As the same with the result of 12 weeks post-implantation, the total point of microscopical examination in FG + MenSCs group was higher than other experimental groups, however, no significant difference was detected between groups at 24 weeks (p > 0.05). Conclusion: In summary, MenSCs as unique stem cell population, is suitable for in vivo repair of OCD defects and promising for the future clinical application.
Background: In recent years, researchers discovered that menstrual blood-derived stem cells (MenSCs) have the potential to differentiate into a wide range of tissues including the chondrogenic lineage. In this study, we aimed to investigate the effect of MenSCs encapsulated in fibrin glue (FG) on healing of osteochondral defect in rabbit model. Methods: We examined the effectiveness of MenSCs encapsulated in FG in comparison with FG alone in the repair of osteochondral defect (OCD) lesions of rabbit knees after 12 and 24 weeks. Results: Macroscopical evaluation revealed that the effectiveness of MenSCs incorporation with FG is much higher than FG alone in repair of OCD defects. Indeed, histopathological evaluation of FG + MenSCs group at 12 weeks post-transplantation demonstrated that defects were filled with hyaline cartilage-like tissue with proper integration, high content of glycosaminoglycan and the existence of collagen fibers especially collagen type II, as well as by passing time (24 weeks post-transplantation), the most regenerated tissue in FG + MenSCs group was similar to hyaline cartilage with relatively good infill and integration. As the same with the result of 12 weeks post-implantation, the total point of microscopical examination in FG + MenSCs group was higher than other experimental groups, however, no significant difference was detected between groups at 24 weeks (p > 0.05). Conclusion: In summary, MenSCs as unique stem cell population, is suitable for in vivo repair of OCD defects and promising for the future clinical application.
Authors: Yvonne E Könst; Rob J Benink; Ron Veldstra; Tjerk J van der Krieke; Marco N Helder; Barend J van Royen Journal: Knee Surg Sports Traumatol Arthrosc Date: 2012-11 Impact factor: 4.342
Authors: Ursula Hopfner; Matthias M Aitzetmueller; Philipp Neßbach; Michael S Hu; Hans-Guenther Machens; Zeshaan N Maan; Dominik Duscher Journal: Stem Cells Int Date: 2018-12-19 Impact factor: 5.443
Authors: Dong Hwan Lee; Chae-Gwan Kong; Yong-Woon Shin; Saif Ahmed; Asode Ananthram Shetty; Myung Sang Moon; Seok Jung Kim Journal: Ann Transl Med Date: 2020-07
Authors: Juan Antonio Rojas-Murillo; Mario A Simental-Mendía; Nidia K Moncada-Saucedo; Paulina Delgado-Gonzalez; José Francisco Islas; Jorge A Roacho-Pérez; Elsa N Garza-Treviño Journal: Int J Mol Sci Date: 2022-08-30 Impact factor: 6.208