Herbert Kaltner1, Gabriel García Caballero1, Fred Sinowatz2, Sebastian Schmidt1, Joachim C Manning1, Sabine André1, Hans-Joachim Gabius3. 1. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539, Munich, Germany. 2. Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539, Munich, Germany. 3. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539, Munich, Germany. Electronic address: gabius@tiph.vetmed.uni-muenchen.de.
Abstract
BACKGROUND: Galectin-related protein (GRP), present in vertebrates, is special within this family of adhesion/growth-regulatory proteins due to its strong positive selection and loss of canonical lectin activity. METHODS: RT-PCR and Western blotting together with flow cytofluorimetry and immunocyto- and histochemistry monitor expression and localization of chicken GRP. The promoter sequence of the GRP gene is processed computationally to detect putative sites for binding transcription factors. The labeled protein is applied as probe to detect binding sites on cells and in sections, along with glycocompounds to test inhibition of the association. RESULTS: Expression of GRP in chicken is limited to bursa of Fabricius, immunohistochemically found in B cells, also in bursal epithelium and vessels. Presence in B cells is shared with only one canonical galectin, i.e. CG-8. Binding to a chicken lymphoma line was specific and saturable, not affected by lactose but completely blocked by heparin, as also seen in sections. CONCLUSIONS: Expression monitoring initiated for GRP reveals a distinct site of localization in chicken, much more restricted than for any of its canonical galectins.
BACKGROUND:Galectin-related protein (GRP), present in vertebrates, is special within this family of adhesion/growth-regulatory proteins due to its strong positive selection and loss of canonical lectin activity. METHODS: RT-PCR and Western blotting together with flow cytofluorimetry and immunocyto- and histochemistry monitor expression and localization of chickenGRP. The promoter sequence of the GRP gene is processed computationally to detect putative sites for binding transcription factors. The labeled protein is applied as probe to detect binding sites on cells and in sections, along with glycocompounds to test inhibition of the association. RESULTS: Expression of GRP in chicken is limited to bursa of Fabricius, immunohistochemically found in B cells, also in bursal epithelium and vessels. Presence in B cells is shared with only one canonical galectin, i.e. CG-8. Binding to a chickenlymphoma line was specific and saturable, not affected by lactose but completely blocked by heparin, as also seen in sections. CONCLUSIONS: Expression monitoring initiated for GRP reveals a distinct site of localization in chicken, much more restricted than for any of its canonical galectins.
Authors: Herbert Kaltner; Gabriel García Caballero; Anna-Kristin Ludwig; Joachim C Manning; Hans-Joachim Gabius Journal: Histochem Cell Biol Date: 2018-05-05 Impact factor: 4.304
Authors: Herbert Kaltner; Stefan Toegel; Gabriel García Caballero; Joachim C Manning; Robert W Ledeen; Hans-Joachim Gabius Journal: Histochem Cell Biol Date: 2016-12-24 Impact factor: 4.304
Authors: Adam Balic; Cosmin Chintoan-Uta; Prerna Vohra; Kate M Sutton; Robin L Cassady-Cain; Tuan Hu; David S Donaldson; Mark P Stevens; Neil A Mabbott; David A Hume; Helen M Sang; Lonneke Vervelde Journal: Front Immunol Date: 2019-10-22 Impact factor: 7.561
Authors: Herbert Kaltner; Joachim C Manning; Gabriel García Caballero; Claudia Di Salvo; Adele Gabba; Laura L Romero-Hernández; Clemens Knospe; Dan Wu; Harrison C Daly; Donal F O'Shea; Hans-Joachim Gabius; Paul V Murphy Journal: RSC Adv Date: 2018-08-14 Impact factor: 4.036
Authors: Gabriel García Caballero; Andrea Flores-Ibarra; Malwina Michalak; Nailya Khasbiullina; Nicolai V Bovin; Sabine André; Joachim C Manning; Sabine Vértesy; Federico M Ruiz; Herbert Kaltner; Jürgen Kopitz; Antonio Romero; Hans-Joachim Gabius Journal: Biochim Biophys Acta Date: 2016-06-03