Andrew M Holwerda1, Imre Wk Kouw1, Jorn Trommelen1, Shona L Halson2, Will Kwh Wodzig3, Lex B Verdijk1, Luc Jc van Loon4. 1. NUTRIM School of Nutrition and Translational Research in Metabolism and Top Institute Food and Nutrition (TIFN), Wageningen, Netherlands; and. 2. AIS Physiology, Australian Institute of Sport, Belconnen, Australia. 3. Central Diagnostic Laboratory, Maastricht University Medical Centre+, Maastricht, Netherlands; 4. NUTRIM School of Nutrition and Translational Research in Metabolism and Top Institute Food and Nutrition (TIFN), Wageningen, Netherlands; and l.vanloon@maastrichtuniversity.nl.
Abstract
BACKGROUND: The age-related decline in skeletal muscle mass is partly attributed to anabolic resistance to food intake. Dietary protein ingestion before sleep could be used as a nutritional strategy to compensate for anabolic resistance. OBJECTIVE: The present study assessed whether physical activity performed in the evening can augment the overnight muscle protein synthetic response to presleep protein ingestion in older men. METHODS: In a parallel group design, 23 healthy older men (mean ± SEM age: 71 ± 1 y) were randomly assigned to ingest 40 g protein intrinsically labeled with l-[1-(13)C]-phenylalanine and l-[1-(13)C]-leucine before going to sleep with (PRO+EX) or without (PRO) performing physical activity earlier in the evening. Overnight protein digestion and absorption kinetics and myofibrillar protein synthesis rates were assessed by combining primed, continuous infusions of l-[ring-(2)H5]-phenylalanine, l-[1-(13)C]-leucine, and l-[ring-(2)H2]-tyrosine with the ingestion of intrinsically labeled casein protein. Muscle and blood samples were collected throughout overnight sleep. RESULTS: Protein ingested before sleep was normally digested and absorbed, with 54% ± 2% of the protein-derived amino acids appearing in the circulation throughout overnight sleep. Overnight myofibrillar protein synthesis rates were 31% (0.058% ± 0.002%/h compared with 0.044% ± 0.003%/h; P < 0.01; based on l-[ring-(2)H5]-phenylalanine) and 27% (0.074% ± 0.004%/h compared with 0.058% ± 0.003%/h; P < 0.01; based on l-[1-(13)C]-leucine) higher in the PRO+EX than in the PRO treatment. More dietary protein-derived amino acids were incorporated into de novo myofibrillar protein during overnight sleep in PRO+EX than in PRO treatment (0.042 ± 0.002 compared with 0.033 ± 0.002 mole percent excess; P < 0.05). CONCLUSIONS: Physical activity performed in the evening augments the overnight muscle protein synthetic response to presleep protein ingestion and allows more of the ingested protein-derived amino acids to be used for de novo muscle protein synthesis during overnight sleep in older men. This trial was registered at Nederlands Trial Register as NTR3885.
RCT Entities:
BACKGROUND: The age-related decline in skeletal muscle mass is partly attributed to anabolic resistance to food intake. Dietary protein ingestion before sleep could be used as a nutritional strategy to compensate for anabolic resistance. OBJECTIVE: The present study assessed whether physical activity performed in the evening can augment the overnight muscle protein synthetic response to presleep protein ingestion in older men. METHODS: In a parallel group design, 23 healthy older men (mean ± SEM age: 71 ± 1 y) were randomly assigned to ingest 40 g protein intrinsically labeled with l-[1-(13)C]-phenylalanine and l-[1-(13)C]-leucine before going to sleep with (PRO+EX) or without (PRO) performing physical activity earlier in the evening. Overnight protein digestion and absorption kinetics and myofibrillar protein synthesis rates were assessed by combining primed, continuous infusions of l-[ring-(2)H5]-phenylalanine, l-[1-(13)C]-leucine, and l-[ring-(2)H2]-tyrosine with the ingestion of intrinsically labeled casein protein. Muscle and blood samples were collected throughout overnight sleep. RESULTS: Protein ingested before sleep was normally digested and absorbed, with 54% ± 2% of the protein-derived amino acids appearing in the circulation throughout overnight sleep. Overnight myofibrillar protein synthesis rates were 31% (0.058% ± 0.002%/h compared with 0.044% ± 0.003%/h; P < 0.01; based on l-[ring-(2)H5]-phenylalanine) and 27% (0.074% ± 0.004%/h compared with 0.058% ± 0.003%/h; P < 0.01; based on l-[1-(13)C]-leucine) higher in the PRO+EX than in the PRO treatment. More dietary protein-derived amino acids were incorporated into de novo myofibrillar protein during overnight sleep in PRO+EX than in PRO treatment (0.042 ± 0.002 compared with 0.033 ± 0.002 mole percent excess; P < 0.05). CONCLUSIONS: Physical activity performed in the evening augments the overnight muscle protein synthetic response to presleep protein ingestion and allows more of the ingested protein-derived amino acids to be used for de novo muscle protein synthesis during overnight sleep in older men. This trial was registered at Nederlands Trial Register as NTR3885.
Authors: Andrew M Holwerda; Kevin J M Paulussen; Maarten Overkamp; Joy P B Goessens; Irene Fleur Kramer; Will K W H Wodzig; Lex B Verdijk; Luc J C van Loon Journal: J Nutr Date: 2019-02-01 Impact factor: 4.798
Authors: Ralf Jäger; Chad M Kerksick; Bill I Campbell; Paul J Cribb; Shawn D Wells; Tim M Skwiat; Martin Purpura; Tim N Ziegenfuss; Arny A Ferrando; Shawn M Arent; Abbie E Smith-Ryan; Jeffrey R Stout; Paul J Arciero; Michael J Ormsbee; Lem W Taylor; Colin D Wilborn; Doug S Kalman; Richard B Kreider; Darryn S Willoughby; Jay R Hoffman; Jamie L Krzykowski; Jose Antonio Journal: J Int Soc Sports Nutr Date: 2017-06-20 Impact factor: 5.150
Authors: Alistair J Monteyne; Mandy V Dunlop; David J Machin; Mariana O C Coelho; George F Pavis; Craig Porter; Andrew J Murton; Doaa R Abdelrahman; Marlou L Dirks; Francis B Stephens; Benjamin T Wall Journal: Br J Nutr Date: 2020-11-11 Impact factor: 3.718