Mariame A Hassan1,2, Yukihiro Furusawa1, Qing-Li Zhao1, Ichiro Takasaki3, Loreto B Feril4, Katsuro Tachibana4, Nobuki Kudo5, Masami Minemura6, Toshiro Sugiyama6, Takashi Kondo7. 1. Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan. 2. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr Al-Aini str., 11562, Cairo, Egypt. 3. Division of Molecular Genetic Research, Life Science Research Center, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan. 4. Department of Anatomy, School of Medicine, Fukuoka University, Nanakuma, Fukuoka, 814-0180, Japan. 5. Laboratory of Biomedical Instrumentation and Measurement, Graduate School of Information Science and Technology, Hokkaido University, Sapporo, 060-0814, Japan. 6. Department of Gastroenterology and Hematology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan. 7. Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan. kondot@med.u-toyama.ac.jp.
Abstract
PURPOSE: Although sanazole has been used as a hypoxic radiosensitizer, we recently reported on its ability to sensitize U937 cells to hyperthermia and X-irradiation under aerobic conditions, enhancing apoptotic cell death following the combined treatment. The current study was undertaken to evaluate the effect of sanazole as a sonosensitizer under previously studied acoustic conditions of different pulse repetition frequencies, using two cell lines representative of solid tumours and haematopoietic cancers. METHODS: Cells were treated with different doses of sanazole. Flow-cytometric analysis and DNA fragmentation assay were carried out at different times, and morphological features were also inspected. For ultrasound treatment, cells were pre-incubated with a non-cytotoxic dose of sanazole for 30 min before exposure. Evaluation of cell killing and a parallel examination of intracellular oxidative stress levels in both cell lines were performed using flow cytometry. RESULTS: Sanazole alone displayed selective cytotoxic effects towards solid tumour-derived cancer cells, resulting in complete cell death after 24 h of treatment, and enhanced the ultrasound-induced cell killing 6 h post-treatment. The enhancement seemed to be mediated by an additive increase in intracellular oxidative stress levels. CONCLUSION: Sanazole seems to be an efficient cytotoxic agent for the treatment of solid tumours and a promising sonosensitizer under aerobic conditions.
PURPOSE: Although sanazole has been used as a hypoxic radiosensitizer, we recently reported on its ability to sensitize U937 cells to hyperthermia and X-irradiation under aerobic conditions, enhancing apoptotic cell death following the combined treatment. The current study was undertaken to evaluate the effect of sanazole as a sonosensitizer under previously studied acoustic conditions of different pulse repetition frequencies, using two cell lines representative of solid tumours and haematopoietic cancers. METHODS: Cells were treated with different doses of sanazole. Flow-cytometric analysis and DNA fragmentation assay were carried out at different times, and morphological features were also inspected. For ultrasound treatment, cells were pre-incubated with a non-cytotoxic dose of sanazole for 30 min before exposure. Evaluation of cell killing and a parallel examination of intracellular oxidative stress levels in both cell lines were performed using flow cytometry. RESULTS:Sanazole alone displayed selective cytotoxic effects towards solid tumour-derived cancer cells, resulting in complete cell death after 24 h of treatment, and enhanced the ultrasound-induced cell killing 6 h post-treatment. The enhancement seemed to be mediated by an additive increase in intracellular oxidative stress levels. CONCLUSION:Sanazole seems to be an efficient cytotoxic agent for the treatment of solid tumours and a promising sonosensitizer under aerobic conditions.
Authors: Bin Yan; Huili Wang; Yuanlin Peng; Ye Hu; He Wang; Xiuwu Zhang; Qi Chen; Joel S Bedford; Mark W Dewhirst; Chuan-Yuan Li Journal: Proc Natl Acad Sci U S A Date: 2006-01-23 Impact factor: 11.205
Authors: S Wada; Z-G Cui; T Kondo; Q-L Zhao; R Ogawa; M Shoji; T Arai; K Makino; I Furuta Journal: Int J Hyperthermia Date: 2005-05 Impact factor: 3.914
Authors: Mikhail A Buldakov; Mariame A Hassan; Qing-Li Zhao; Loreto B Feril; Nobuki Kudo; Takashi Kondo; Nikolai V Litvyakov; Mikhail A Bolshakov; Vladislav V Rostov; Nadejda V Cherdyntseva; Peter Riesz Journal: Ultrason Sonochem Date: 2008-10-17 Impact factor: 7.491