Andrew J Ocque1, Colleen E Hagler1, Robin Difrancesco1, Yvonne Woolwine-Cunningham2, Cindy J Bednasz1, Gene D Morse1, Andrew H Talal3. 1. Translational Pharmacology Research Core, Department of Pharmacy Practice, School of Pharmacy & Pharmaceutical Sciences, New York State Center of Excellence in Bioinformatics & Life Sciences, University at Buffalo, Buffalo, NY, USA. 2. Clinical & Translational Research Center, University at Buffalo, Buffalo, NY, USA. 3. Division of Gastroenterology & Hepatology & Center for Clinical & Research in Liver Disease, Jacobs School of Medicine, University at Buffalo, Buffalo, NY, USA.
Abstract
AIM: Determination of paritaprevir and ritonavir in rat liver tissue samples. RESULTS: We successfully validated a UPLC-MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards (d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study. CONCLUSION: The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.
AIM: Determination of paritaprevir and ritonavir in rat liver tissue samples. RESULTS: We successfully validated a UPLC-MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards (d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study. CONCLUSION: The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.
Authors: R E Aarnoutse; C P Verweij-van Wissen; W J Underberg; J Kleinnijenhuis; Y A Hekster; D M Burger Journal: J Chromatogr B Biomed Sci Appl Date: 2001-11-25
Authors: John DeWitt; Chang-Min Cho; Jingmei Lin; Mohammad Al-Haddad; Marcia Irene Canto; Ashley Salamone; Ralph H Hruban; Ahmed A Messallam; Mouen A Khashab Journal: Endosc Int Open Date: 2015-06-24
Authors: Charles S Venuto; Marianthi Markatou; Yvonne Woolwine-Cunningham; Rosemary Furlage; Andrew J Ocque; Robin DiFrancesco; Emily O Dumas; Paul K Wallace; Gene D Morse; Andrew H Talal Journal: Antimicrob Agents Chemother Date: 2017-04-24 Impact factor: 5.191
Authors: Andrew J Ocque; Colleen E Hagler; Robin DiFrancesco; Gene D Morse; Andrew H Talal Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2017-03-24 Impact factor: 3.205
Authors: Andrew H Talal; Emily O Dumas; Barbara Bauer; Richard M Rejman; Andrew Ocque; Gene D Morse; Danijela Lucic; Gavin A Cloherty; Jennifer King; Jiuhong Zha; Hongtao Zhang; Daniel E Cohen; Nancy Shulman; Jean-Michel Pawlotsky; Christophe Hézode Journal: J Infect Dis Date: 2018-01-17 Impact factor: 5.226
Authors: Maria A Acquavia; Luca Foti; Raffaella Pascale; Antonia Nicolò; Vincenzo Brancaleone; Tommaso R I Cataldi; Giuseppe Martelli; Laura Scrano; Giuliana Bianco Journal: Talanta Date: 2020-11-05 Impact factor: 6.057