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Literature DB >> 272652

Diadenosine 5',5'''-P1,P4-tetraphosphate triggers initiation of in vitro DNA replication in baby hamster kidney cells.

Abstract

Addition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to permeabilized G1-arrested baby hamster kidney cells resulted in the stimulation of DNA synthesis. No stimulation was observed in cells from exponentially growing cultures. The Ap4A-stimulated [3H]dTTP incorporation was inhibited by nalidixic acid, daunomycin, chloroquine diphosphate, EDTA, and N-ethylmaleimide. It was dose-dependent in regard to the amount of permeabilized cells and of Ap4A. Numerous replication eyes were formed in the DNA molecules of stimulated cells. Pulse-chase experiments showed that the synthesis of DNA was discontinuous, resulting in the appearance of approximately 4S Okazaki fragments and their ligation to high molecular weight DNA. These results strongly suggest that Ap4A stimulated the initiation of DNA synthesis in baby hamster kidney cells that had been arrested in G1 by serum deprival.

Entities: Chemical Species

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Year: 1978 PMID： 272652 PMCID： PMC411250 DOI： 10.1073/pnas.75.1.371

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

29 in total

1. Presence of diadenosine 5',5''' -P1, P4-tetraphosphate (Ap4A) in mamalian cells in levels varying widely with proliferative activity of the tissue: a possible positive "pleiotypic activator".

Authors: E Rapaport; P C Zamecnik
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

2. Regulation of ATP pools, rRNA and DNA synthesis in 3T3 cells in response to serum or hypoxanthine.

Authors: F Grummt; D Paul; I Grummt
Journal: Eur J Biochem Date: 1977-06-01

3. Control of nucleolar RNA synthesis by the intracellular pool sizes of ATP and GTP.

Authors: I Grummt; F Grummt
Journal: Cell Date: 1976-03 Impact factor: 41.582

4. Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.

Authors: E L Winnacker; G Magnusson; P Reichard
Journal: J Mol Biol Date: 1972-12-30 Impact factor: 5.469

5. Isolation and characterization of dinucleoside tetra- and tri-phosphates formed in the presence of lysyl-sRNA synthetase.

Authors: K Randerath; C M Janeway; M L Stephenson; P C Zamecnik
Journal: Biochem Biophys Res Commun Date: 1966-07-06 Impact factor: 3.575

6. Initiation of DNA synthesis in HeLa cell-free system.

Authors: K V Kumar; D L Friedman
Journal: Nat New Biol Date: 1972-09-20

7. Stimulation of nuclear DNA and RNA synthesis by cytoplasmic extracts in vitro.

Authors: L R Thompson; B J McCarthy
Journal: Biochem Biophys Res Commun Date: 1968-01-25 Impact factor: 3.575

8. Effect of cytosol on DNA synthesis in isolated HeLa cell nuclei.

Authors: H Krokan; E Wist; H Prydz
Journal: Biochem Biophys Res Commun Date: 1977-03-21 Impact factor: 3.575

9. Acitivity of yeast extracts in cell-free stimulation of DNA replication.

Authors: S M Jazwinski; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

10. Stimulation of sea urchin DNA polymerase by protein factors. II. Formation of active complex in DNA polymerase reaction.

Authors: K Murakami-Murofushi; Y Mano
Journal: Biochim Biophys Acta Date: 1977-03-18

33 in total

1. Purification, immunological and biochemical characterization of Ap4A binding protein from Xenopus laevis oocytes.

Authors: L Zourgui; D Baltz; T Baltz; F Oukerro; L Tarrago-Litvak
Journal: Nucleic Acids Res Date: 1988-04-11 Impact factor: 16.971

2. Transfer RNA-like structure of the human Alu family: implications of its generation mechanism and possible functions.

Authors: N Okada
Journal: J Mol Evol Date: 1990-12 Impact factor: 2.395

3. Diadenosine tetraphosphate (Ap4A): a putative chemical messenger of cell proliferation control and inducer of DNA replication : Review paper.

Authors: F Grummt
Journal: Plant Mol Biol Date: 1983-01 Impact factor: 4.076

4. Studies on some specific Ap4A-degrading enzymes with the use of various methylene analogues of P1P4-bis-(5',5'''-adenosyl) tetraphosphate.

Authors: A Guranowski; E Starzyńska; G E Taylor; G M Blackburn
Journal: Biochem J Date: 1989-08-15 Impact factor: 3.857

5. Synthesis and resistance to enzymic hydrolysis of stereochemically-defined phosphonate and thiophosphate analogues of P1,P4-bis(5'-adenosyl) tetraphosphate.

Authors: G M Blackburn; G E Taylor; G R Thatcher; M Prescott; A G McLennan
Journal: Nucleic Acids Res Date: 1987-09-11 Impact factor: 16.971

6. In vitro replication of recombinant plasmids carrying chromosomal segments of Xenopus laevis.

Authors: S Hiraga; T Sudo; M Yoshida; H Kubota; H Ueyama
Journal: Proc Natl Acad Sci U S A Date: 1982-06 Impact factor: 11.205

7. Acyl coenzyme A synthetase from Pseudomonas fragi catalyzes the synthesis of adenosine 5'-polyphosphates and dinucleoside polyphosphates.

Authors: R Fontes; M A Sillero; A Sillero
Journal: J Bacteriol Date: 1998-06 Impact factor: 3.490

8. In vivo levels of diadenosine tetraphosphate and adenosine tetraphospho-guanosine in Physarum polycephalum during the cell cycle and oxidative stress.

Authors: P N Garrison; S A Mathis; L D Barnes
Journal: Mol Cell Biol Date: 1986-04 Impact factor: 4.272

Review 9. The search for guanosine tetraphosphate (ppGpp) and other unusual nucleotides in eucaryotes.

Authors: R H Silverman; A G Atherly
Journal: Microbiol Rev Date: 1979-03

10. Diadenosine 5',5'''-P1,P4-tetraphosphate, a ligand of the 57-kilodalton subunit of DNA polymerase alpha.

Authors: F Grummt; G Waltl; H M Jantzen; K Hamprecht; U Huebscher; C C Kuenzle
Journal: Proc Natl Acad Sci U S A Date: 1979-12 Impact factor: 11.205