Amira Abdel Motaal1,2, Shahira M Ezzat1, Mariane G Tadros3, Hesham I El-Askary1. 1. a Department of Pharmacognosy, Faculty of Pharmacy , Cairo University , Cairo , Egypt. 2. b Pharmaceutical Biology Department, Faculty of Pharmacy and Biotechnology , GUC , Cairo , Egypt. 3. c Department of Pharmacology, Faculty of Pharmacy , Ein Shams University , Cairo , Egypt.
Abstract
CONTEXT: Solidago virgaurea L. (Asteraceae) is traditionally used as an anti-inflammatory for the treatment of various symptoms including cystitis. However, little is known concerning the constituents responsible for this activity and the mechanism of their action. OBJECTIVE: To assess the anti-inflammatory activity of the phenolic-rich fraction of S. virgaurea aerial parts in rats, isolate and assess the activity of the major compounds present. MATERIALS AND METHODS: An HPLC method was developed for the analysis of the phenolic-rich fraction (EtFr). The in vivo anti-inflammatory activity of the EtFr and four isolated compounds (at 25 and 50 mg/kg) were assessed in adult male rats using the carrageenan-induced rat paw oedema model. The levels of the pro-inflammatory cytokines (TNF-α and IL-1β) were measured using ELISA. RESULTS: 3,5-O-Dicaffeoylquinic acid (1), 3,4-O-dicaffeoylquinic acid (2), 3,4,5-O-tricaffeoylquinic acid (3) and 4,5-O-dicaffeoylquinic acid (4) were isolated from EtFr. Compound 3 (50 mg/kg) showed a highly significant activity in inhibiting the oedema volume after 3 h (88% of the activity of indomethacin at 10 mg/kg). The EtFr and the isolated compounds largely inhibited the excessive production of the inflammatory mediators TNF-α and IL-1β. DISCUSSION AND CONCLUSION: This is the first report of 3,4,5-tri-O-caffeoylquinic acid (3) in Solidago species. The tricaffeoylquinic acid (3) showed a significantly higher activity than the other three dicaffeoylquinic acids (1, 2, 4) and indomethacin in reduction of TNF-α and IL-1β concentrations (8.44 ± 0.62 and 5.83 ± 0.57 pg/mL compared to 12.60 ± 1.30 and 52.91 ± 5.20 pg/mL induced by indomethacin, respectively).
CONTEXT: Solidago virgaurea L. (Asteraceae) is traditionally used as an anti-inflammatory for the treatment of various symptoms including cystitis. However, little is known concerning the constituents responsible for this activity and the mechanism of their action. OBJECTIVE: To assess the anti-inflammatory activity of the phenolic-rich fraction of S. virgaurea aerial parts in rats, isolate and assess the activity of the major compounds present. MATERIALS AND METHODS: An HPLC method was developed for the analysis of the phenolic-rich fraction (EtFr). The in vivo anti-inflammatory activity of the EtFr and four isolated compounds (at 25 and 50 mg/kg) were assessed in adult male rats using the carrageenan-induced ratpaw oedema model. The levels of the pro-inflammatory cytokines (TNF-α and IL-1β) were measured using ELISA. RESULTS:3,5-O-Dicaffeoylquinic acid (1), 3,4-O-dicaffeoylquinic acid (2), 3,4,5-O-tricaffeoylquinic acid (3) and 4,5-O-dicaffeoylquinic acid (4) were isolated from EtFr. Compound 3 (50 mg/kg) showed a highly significant activity in inhibiting the oedema volume after 3 h (88% of the activity of indomethacin at 10 mg/kg). The EtFr and the isolated compounds largely inhibited the excessive production of the inflammatory mediators TNF-α and IL-1β. DISCUSSION AND CONCLUSION: This is the first report of 3,4,5-tri-O-caffeoylquinic acid (3) in Solidago species. The tricaffeoylquinic acid (3) showed a significantly higher activity than the other three dicaffeoylquinic acids (1, 2, 4) and indomethacin in reduction of TNF-α and IL-1β concentrations (8.44 ± 0.62 and 5.83 ± 0.57 pg/mL compared to 12.60 ± 1.30 and 52.91 ± 5.20 pg/mL induced by indomethacin, respectively).
Authors: Xueyun Zheng; Ryan S Renslow; Mpho M Makola; Ian K Webb; Liulin Deng; Dennis G Thomas; Niranjan Govind; Yehia M Ibrahim; Mwadham M Kabanda; Ian A Dubery; Heino M Heyman; Richard D Smith; Ntakadzeni E Madala; Erin S Baker Journal: J Phys Chem Lett Date: 2017-03-15 Impact factor: 6.475
Authors: Abdelsamed I Elshamy; Abdel Razik H Farrag; Iriny M Ayoub; Karam A Mahdy; Rehab F Taher; Abd El-Nasser G Ei Gendy; Tarik A Mohamed; Salim S Al-Rejaie; Yasser A Ei-Amier; Ahmed M Abd-EIGawad; Mohamed A Farag Journal: Molecules Date: 2020-09-15 Impact factor: 4.411