Zeynep Cakar1, Burcu Cetinkaya1, Duru Aras1, Betül Koca1, Sinan Ozkavukcu2, İskender Kaplanoglu3, Alp Can1, Ozgur Cinar4. 1. Department of Histology and Embryology, Laboratories for Stem Cells and Reproductive Biology, Ankara University School of Medicine, Sihhiye, 06100, Ankara, Turkey. 2. Ankara University School of Medicine, Center for Assisted Reproduction, Cebeci, Ankara, Turkey. 3. Etlik Zubeyde Hanim Women's Health Teaching and Research Hospital, Center for Assisted Reproduction, Etlik, Ankara, Turkey. 4. Department of Histology and Embryology, Laboratories for Stem Cells and Reproductive Biology, Ankara University School of Medicine, Sihhiye, 06100, Ankara, Turkey. ocinar@ankara.edu.tr.
Abstract
PURPOSE: The present study aimed to evaluate whether combining the magnetic-activated cell sorting (MACS) with density-gradient (DG) or swim-up (SU) sperm separation techniques can improve sperm selection to obtain higher quality spermatozoa. METHODS: Two commonly used sperm selection techniques, SU and DG, were compared to MACS combined with either SU or DG. Spermatozoa obtained from normozoospermic (n = 10) and oligozoospermic (n = 10) cases were grouped as SU, DG, SU+MACS, and DG+MACS followed by the analysis of sperm morphology, motility, DNA integrity, and the levels of Izumo-1 and PLCZ proteins. RESULTS: Although spermatozoa obtained by SU or DG when combined with MACS have improved aspects when compared to SU or DG alone, results did not reach a statistically significant level. Moreover, separation with MACS caused a significant loss in the numbers of total and rapid progressive spermatozoa. CONCLUSIONS: Considering the cost/benefit ratio, MACS application together with traditional techniques may only be preferred in certain cases having higher concentrations of spermatozoa, but it does not seem to be an ideal and practical sperm selection technique for routine use.
PURPOSE: The present study aimed to evaluate whether combining the magnetic-activated cell sorting (MACS) with density-gradient (DG) or swim-up (SU) sperm separation techniques can improve sperm selection to obtain higher quality spermatozoa. METHODS: Two commonly used sperm selection techniques, SU and DG, were compared to MACS combined with either SU or DG. Spermatozoa obtained from normozoospermic (n = 10) and oligozoospermic (n = 10) cases were grouped as SU, DG, SU+MACS, and DG+MACS followed by the analysis of sperm morphology, motility, DNA integrity, and the levels of Izumo-1 and PLCZ proteins. RESULTS: Although spermatozoa obtained by SU or DG when combined with MACS have improved aspects when compared to SU or DG alone, results did not reach a statistically significant level. Moreover, separation with MACS caused a significant loss in the numbers of total and rapid progressive spermatozoa. CONCLUSIONS: Considering the cost/benefit ratio, MACS application together with traditional techniques may only be preferred in certain cases having higher concentrations of spermatozoa, but it does not seem to be an ideal and practical sperm selection technique for routine use.
Entities:
Keywords:
DNA integrity; Izumo-1; Magnetic cell sorting; PLC-ζ; Sperm selection
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