Literature DB >> 25454495

Morphological evaluation of sperm from infertile men selected by magnetic activated cell sorting (MACS).

Gianni Curti1, Fernanda Skowronek2, Rita Vernochi3, Ana Laura Rodriguez-Buzzi4, Juan Carlos Rodriguez-Buzzi5, Gabriela Casanova6, Rossana Sapiro7.   

Abstract

Electron microscopy analysis performed in five infertile human subjects after sperm selection by swim-up followed by magnetic activated cell sorting (MACS) demonstrated a decrease in the number of spermatozoa with characteristics compatible with cell death. However, no significant differences were found when the swim-up/MACS semen fraction was compared with swim-up fraction alone.
Copyright © 2014 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Entities:  

Keywords:  Annexin V; Apoptotic sperm; DNA damage; Electron microscopy; Magnetic activated cell sorting

Mesh：

Year:  2014        PMID： 25454495     DOI： 10.1016/j.repbio.2014.07.002

Source DB:  PubMed          Journal:  Reprod Biol        ISSN： 1642-431X            Impact factor:   2.376


  3 in total

1.  Magnetic-activated cell sorting of non-apoptotic spermatozoa improves the quality of embryos according to female age: a prospective sibling oocyte study.

Authors:  Martin Stimpfel; Ivan Verdenik; Branko Zorn; Irma Virant-Klun
Journal:  J Assist Reprod Genet       Date:  2018-06-27       Impact factor: 3.412

2.  Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

Authors:  Zeynep Cakar; Burcu Cetinkaya; Duru Aras; Betül Koca; Sinan Ozkavukcu; İskender Kaplanoglu; Alp Can; Ozgur Cinar
Journal:  J Assist Reprod Genet       Date:  2016-05-28       Impact factor: 3.412

3.  MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content: a pilot study.

Authors:  Sahar El Fekih; Nadia Gueganic; Corinne Tous; Habib Ben Ali; Mounir Ajina; Nathalie Douet-Guilbert; Hortense Drapier; Damien Beauvillard; Frédéric Morel; Aurore Perrin
Journal:  Asian J Androl       Date:  2022 Sep-Oct       Impact factor: 3.054
  3 in total

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