| Literature DB >> 27222636 |
Huanan Wang1, Lei Wang2, Yingjie Zhang2, Ji Wang2, Yibin Deng2, Degui Lin3.
Abstract
[This corrects the article DOI: 10.1186/s12935-016-0280-y.].Entities:
Year: 2016 PMID: 27222636 PMCID: PMC4878060 DOI: 10.1186/s12935-016-0313-6
Source DB: PubMed Journal: Cancer Cell Int ISSN: 1475-2867 Impact factor: 5.722
Fig. 1shRNA-mediated knockdown of oncogenic Kras reduces HK2 expression and suppresses growth in NSCLC cell lines. a, b Protein level of Kras and HK2 was detected in KP2 and H23 cells expressing shRNAs for Kras. c, d Clonogenic survival assays were performed to assess cell growth. Colonies were stained by crystal violet after 7 days of cell growth. e, f Xenograft tumor growth. 1 × 106 cells expressing scramble shRNA (CON) or shRNA for Kras were subcutaneously injected to the lower flank of NSG mice. Representative images of tumors at 4 weeks after injection are shown (e). Quantification of tumors weight (P < 0.01) (f). g IHC staining of cell proliferation marker Ki67 from control or Kras-knockdown KP2 cells. h Quantification of Ki67 expression from representative images shown in g (P < 0.01)