| Literature DB >> 27212708 |
Qingling Xu1, Cheol Ho Heo2, Jin A Kim1, Hye Sue Lee2, Ying Hu1, Dayoung Kim1, Kunemadihalli Mathada Kotraiah Swamy1,3, Gyoungmi Kim1, Sang-Jip Nam1, Hwan Myung Kim2, Juyoung Yoon1.
Abstract
Hypochlorite (OCl(-)) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl(-) at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl(-) recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl(-) in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl(-), displayed a selective fluorescent off/on response to OCl(-) with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl(-) in live RAW 264.7 cells via two-photon microscopy.Entities:
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Year: 2016 PMID： 27212708 DOI： 10.1021/acs.analchem.6b01738
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986