Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation.

Literature DB >> 27210041

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation.

Wenbo Wang¹, Peter M Kutny¹, Shannon L Byers¹, Charles J Longstaff¹, Michael J DaCosta¹, Changhong Pang¹, Yingfan Zhang¹, Robert A Taft¹, Frank W Buaas¹, Haoyi Wang².

Abstract

Previously we established Zygote Electroporation of Nucleases (ZEN) technology as an efficient and high-throughput way to generate genetically modified mouse models. However, there were significant variations of the targeting efficiency among different genomic loci using our previously published protocol. In this study, we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of electroporation. Using this approach, we were able to introduce precise nucleotide substitutions, large segment deletion and short segment insertion into targeted loci with high efficiency.

Entities: Chemical Disease Gene Species

Keywords: CRISPR-Cas9; Electroporation; Mouse zygote

Mesh：

Substances：

Year: 2016 PMID： 27210041 PMCID： PMC4892940 DOI： 10.1016/j.jgg.2016.02.004

Source DB: PubMed Journal: J Genet Genomics ISSN： 1673-8527 Impact factor: 4.275

22 in total

1. Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized cas9 and evaluation of off-targeting effect.

Authors: Da Liu; Zhanxiang Wang; An Xiao; Yutian Zhang; Wenyuan Li; Yao Zu; Shaohua Yao; Shuo Lin; Bo Zhang
Journal: J Genet Genomics Date: 2013-11-22 Impact factor: 4.275

2. Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs).

Authors: Shannon L Byers; Suzan J Payson; Rob A Taft
Journal: Theriogenology Date: 2005-11-04 Impact factor: 2.740

3. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos.

Authors: Yuyu Niu; Bin Shen; Yiqiang Cui; Yongchang Chen; Jianying Wang; Lei Wang; Yu Kang; Xiaoyang Zhao; Wei Si; Wei Li; Andy Peng Xiang; Jiankui Zhou; Xuejiang Guo; Ye Bi; Chenyang Si; Bian Hu; Guoying Dong; Hong Wang; Zuomin Zhou; Tianqing Li; Tao Tan; Xiuqiong Pu; Fang Wang; Shaohui Ji; Qi Zhou; Xingxu Huang; Weizhi Ji; Jiahao Sha
Journal: Cell Date: 2014-01-30 Impact factor: 41.582

4. Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells.

Authors: Karen M Chapman; Gerardo A Medrano; Priscilla Jaichander; Jaideep Chaudhary; Alexandra E Waits; Marcelo A Nobrega; James M Hotaling; Carole Ober; F Kent Hamra
Journal: Cell Rep Date: 2015-03-12 Impact factor: 9.423

5. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins.

Authors: Kathrin Schumann; Steven Lin; Eric Boyer; Dimitre R Simeonov; Meena Subramaniam; Rachel E Gate; Genevieve E Haliburton; Chun J Ye; Jeffrey A Bluestone; Jennifer A Doudna; Alexander Marson
Journal: Proc Natl Acad Sci U S A Date: 2015-07-27 Impact factor: 11.205

6. Genome-wide inactivation of porcine endogenous retroviruses (PERVs).

Authors: Luhan Yang; Marc Güell; Dong Niu; Haydy George; Emal Lesha; Dennis Grishin; John Aach; Ellen Shrock; Weihong Xu; Jürgen Poci; Rebeca Cortazio; Robert A Wilkinson; Jay A Fishman; George Church
Journal: Science Date: 2015-10-11 Impact factor: 47.728

7. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

Authors: Haoyi Wang; Hui Yang; Chikdu S Shivalila; Meelad M Dawlaty; Albert W Cheng; Feng Zhang; Rudolf Jaenisch
Journal: Cell Date: 2013-05-02 Impact factor: 41.582

8. Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype.

Authors: Hao Yin; Wen Xue; Sidi Chen; Roman L Bogorad; Eric Benedetti; Markus Grompe; Victor Koteliansky; Phillip A Sharp; Tyler Jacks; Daniel G Anderson
Journal: Nat Biotechnol Date: 2014-03-30 Impact factor: 54.908

9. Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

Authors: Wenning Qin; Stephanie L Dion; Peter M Kutny; Yingfan Zhang; Albert W Cheng; Nathaniel L Jillette; Ankit Malhotra; Aron M Geurts; Yi-Guang Chen; Haoyi Wang
Journal: Genetics Date: 2015-03-27 Impact factor: 4.562

10. Simple knockout by electroporation of engineered endonucleases into intact rat embryos.

Authors: Takehito Kaneko; Tetsushi Sakuma; Takashi Yamamoto; Tomoji Mashimo
Journal: Sci Rep Date: 2014-10-01 Impact factor: 4.379

32 in total

Review 1. Gene-edited CRISPy Critters for alcohol research.

Authors: Gregg E Homanics
Journal: Alcohol Date: 2018-03-07 Impact factor: 2.405

2. Electroporation of AsCpf1/RNP at the Zygote Stage is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor.

Authors: Yeon Sun Kim; Gyeong Ryeong Kim; Mira Park; Seung Chel Yang; So Hee Park; Ji Eun Won; Ju Hee Lee; Ha Eun Shin; Haengseok Song; Hye-Ryun Kim
Journal: Tissue Eng Regen Med Date: 2019-11-19 Impact factor: 4.169

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation.

1. Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized cas9 and evaluation of off-targeting effect.

2. Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs).

3. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos.

4. Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells.

5. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins.

6. Genome-wide inactivation of porcine endogenous retroviruses (PERVs).

7. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

8. Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype.

9. Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

10. Simple knockout by electroporation of engineered endonucleases into intact rat embryos.

Review 1. Gene-edited CRISPy Critters for alcohol research.

2. Electroporation of AsCpf1/RNP at the Zygote Stage is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor.

Review 3. Cornerstones of CRISPR-Cas in drug discovery and therapy.

Review 4. Messenger RNA Delivery for Tissue Engineering and Regenerative Medicine Applications.

5. CRISPRtools: a flexible computational platform for performing CRISPR/Cas9 experiments in the mouse.

6. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.

7. CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection.

8. Efficient mouse genome engineering by CRISPR-EZ technology.

Review 9. CRISPR/Cas9 ribonucleoprotein-mediated genome and epigenome editing in mammalian cells.

Review 10. Methods for CRISPR-Cas as Ribonucleoprotein Complex Delivery In Vivo.