Elena Díaz-Santiago1, Luis Rodríguez-Caso1, Casimiro Cárdenas1,2, José J Serrano1, Ana R Quesada1,3, Miguel Ángel Medina1,3. 1. a Departamento de Biología Molecular y Bioquímica , Facultad de Ciencias, and IBIMA (Biomedical Research Institute of Málaga), Universidad de Málaga , Andalucía Tech , Spain. 2. b Research Support Central Services (SCAI) of the University of Málaga , Spain. 3. c CIBER de Enfermedades Raras (CIBERER) , E-29071 Málaga , Spain.
Abstract
OBJECTIVE: We studied the modulatory effects of homocysteine pre-treatment on the disulfide reduction capacity of tumor and endothelial cells. METHODS: Human MDA-MB-231 breast carcinoma and bovine aorta endothelial cells were pre-treated for 1-24 hours with 0.5-5 mM homocysteine or homocysteine thiolactone. After washing to eliminate any rest of homocysteine or homocysteine thiolactone, cell redox capacity was determined by using a method for measuring disulfide reduction. RESULTS: Homocysteine pre-treatments for 1-4 hours at a concentration of 0.5-5 mM increase the disulfide reduction capacity of both tumor and endothelial cells. This effect cannot be fully mimicked by either cysteine or homocysteine thiolactone pre-treatments of tumor cells. DISCUSSION: Taken together, our data suggest that homocysteine can behave as an anti-oxidant agent by increasing the anti-oxidant capacity of tumor and endothelial cells.
OBJECTIVE: We studied the modulatory effects of homocysteine pre-treatment on the disulfide reduction capacity of tumor and endothelial cells. METHODS:Human MDA-MB-231 breast carcinoma and bovine aorta endothelial cells were pre-treated for 1-24 hours with 0.5-5 mM homocysteine or homocysteinethiolactone. After washing to eliminate any rest of homocysteine or homocysteinethiolactone, cell redox capacity was determined by using a method for measuring disulfide reduction. RESULTS:Homocysteine pre-treatments for 1-4 hours at a concentration of 0.5-5 mM increase the disulfide reduction capacity of both tumor and endothelial cells. This effect cannot be fully mimicked by either cysteine or homocysteine thiolactone pre-treatments of tumor cells. DISCUSSION: Taken together, our data suggest that homocysteine can behave as an anti-oxidant agent by increasing the anti-oxidant capacity of tumor and endothelial cells.
Entities:
Keywords:
Bovine aortic endothelial cells; Cysteine; Homocysteine; Homocysteine thiolactone; MDA-MB231 breast cancer cell; Redox
Authors: D E Wilcken; X L Wang; T Adachi; H Hara; N Duarte; K Green; B Wilcken Journal: Arterioscler Thromb Vasc Biol Date: 2000-05 Impact factor: 8.311