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Literature DB >> 27196944

Crystal Structure of Hypusine-Containing Translation Factor eIF5A Bound to a Rotated Eukaryotic Ribosome.

Sergey Melnikov^1,2, Justine Mailliot^1,2, Byung-Sik Shin³, Lukas Rigger⁴, Gulnara Yusupova^1,2, Ronald Micura⁴, Thomas E Dever³, Marat Yusupov^1,2.

Abstract

Eukaryotic translation initiation factor eIF5A promotes protein synthesis by resolving polyproline-induced ribosomal stalling. Here, we report a 3.25-Å resolution crystal structure of eIF5A bound to the yeast 80S ribosome. The structure reveals a previously unseen conformation of an eIF5A-ribosome complex and highlights a possible functional link between conformational changes of the ribosome during protein synthesis and the eIF5A-ribosome association.

Entities: Chemical

Keywords: crystallography; eIF5A; hypusine; ribosome; structure

Mesh：

Substances：

Year: 2016 PMID： 27196944 PMCID： PMC5408928 DOI： 10.1016/j.jmb.2016.05.011

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

18 in total

1. A paralog of lysyl-tRNA synthetase aminoacylates a conserved lysine residue in translation elongation factor P.

Authors: Tatsuo Yanagisawa; Tomomi Sumida; Ryohei Ishii; Chie Takemoto; Shigeyuki Yokoyama
Journal: Nat Struct Mol Biol Date: 2010-08-22 Impact factor: 15.369

2. Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex.

Authors: David Li-En Jao; Kuang Yu Chen
Journal: J Cell Biochem Date: 2006-02-15 Impact factor: 4.429

3. eIF5A binds to translational machinery components and affects translation in yeast.

Authors: Cleslei F Zanelli; Ana L C Maragno; Ana P B Gregio; Suzanne Komili; José R Pandolfi; Carlos A Mestriner; Wilton R Lustri; Sandro R Valentini
Journal: Biochem Biophys Res Commun Date: 2006-08-07 Impact factor: 3.575

Review 4. The hypusine-containing translation factor eIF5A.

Authors: Thomas E Dever; Erik Gutierrez; Byung-Sik Shin
Journal: Crit Rev Biochem Mol Biol Date: 2014-07-17 Impact factor: 8.250

5. Post-translational formation of hypusine: biogenesis of translation initiation factor eIF-4D.

Authors: M H Park; A Abbruzzese; J E Folk
Journal: Adv Exp Med Biol Date: 1988 Impact factor: 2.622

6. Translation elongation factor EF-P alleviates ribosome stalling at polyproline stretches.

Authors: Susanne Ude; Jürgen Lassak; Agata L Starosta; Tobias Kraxenberger; Daniel N Wilson; Kirsten Jung
Journal: Science Date: 2012-12-13 Impact factor: 47.728

7. eIF5A promotes translation of polyproline motifs.

Authors: Erik Gutierrez; Byung-Sik Shin; Christopher J Woolstenhulme; Joo-Ran Kim; Preeti Saini; Allen R Buskirk; Thomas E Dever
Journal: Mol Cell Date: 2013-05-30 Impact factor: 17.970

8. Identification of the hypusine-containing protein hy+ as translation initiation factor eIF-4D.

Authors: H L Cooper; M H Park; J E Folk; B Safer; R Braverman
Journal: Proc Natl Acad Sci U S A Date: 1983-04 Impact factor: 11.205

9. Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome.

Authors: Gregor Blaha; Robin E Stanley; Thomas A Steitz
Journal: Science Date: 2009-08-21 Impact factor: 47.728

10. Hypusine-containing protein eIF5A promotes translation elongation.

Authors: Preeti Saini; Daniel E Eyler; Rachel Green; Thomas E Dever
Journal: Nature Date: 2009-05-07 Impact factor: 49.962

24 in total

1. Translation Initiation Site Profiling Reveals Widespread Synthesis of Non-AUG-Initiated Protein Isoforms in Yeast.

Authors: Amy R Eisenberg; Andrea L Higdon; Ina Hollerer; Alexander P Fields; Irwin Jungreis; Paige D Diamond; Manolis Kellis; Marko Jovanovic; Gloria A Brar
Journal: Cell Syst Date: 2020-07-24 Impact factor: 10.304

2. Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis.

Authors: Andrey G Malyutin; Sharmishtha Musalgaonkar; Stephanie Patchett; Joachim Frank; Arlen W Johnson
Journal: EMBO J Date: 2017-02-08 Impact factor: 11.598

Review 3. Translation elongation factor P (EF-P).

Authors: Katherine R Hummels; Daniel B Kearns
Journal: FEMS Microbiol Rev Date: 2020-03-01 Impact factor: 16.408

4. Regulation of gene expression by translation factor eIF5A: Hypusine-modified eIF5A enhances nonsense-mediated mRNA decay in human cells.

Authors: Mainul Hoque; Ji Yeon Park; Yun-Juan Chang; Augusto D Luchessi; Tavane D Cambiaghi; Raghavendra Shamanna; Hartmut M Hanauske-Abel; Bart Holland; Tsafi Pe'ery; Bin Tian; Michael B Mathews
Journal: Translation (Austin) Date: 2017-08-14

5. Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing.

Authors: Ivaylo P Ivanov; Byung-Sik Shin; Gary Loughran; Ioanna Tzani; Sara K Young-Baird; Chune Cao; John F Atkins; Thomas E Dever
Journal: Mol Cell Date: 2018-04-19 Impact factor: 17.970

Review 6. Roles of polyamines in translation.

Authors: Thomas E Dever; Ivaylo P Ivanov
Journal: J Biol Chem Date: 2018-10-15 Impact factor: 5.157