Elisa Cerri1, Nicola Origlia1, Benedetto Falsini2, Davide Barloscio1, Carlotta Fabiani1, Marco Sansò3, Sara Ottino3, Luca Giovannini4, Luciano Domenici5. 1. Neuroscience Institute of the National Council of Research (CNR), Pisa, Italy. 2. Institute of Ophthalmology, Policlinico Gemelli, Catholic University, Rome, Italy. 3. ITH S.r.l., Pisa, Italy. 4. Department of Translational Research on New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy. 5. Neuroscience Institute of the National Council of Research (CNR), Pisa, Italy ; Department of Biotechnological and Applied Clinical Sciences (DISCAB), University of L'Aquila, L'Aquila, Italy.
Abstract
PURPOSE: To test whether the topical eye treatment with BDNF prevents the effects of continuous light exposure (LE) in the albino rat retina. METHODS: Two groups of albino rats were used. The first group of rats received an intraocular injection of BDNF (2 μL, 1 μg/μL) before LE, while the second group was treated with one single drop of BDNF (10 μL, 12 μg/μL) dissolved in different types of solutions (physiological solution, the polysaccharide fraction of Tamarind gum, TSP, and sodium carboxy methyl cellulose), at the level of conjunctival fornix before LE. The level of BDNF in the retina and optic nerve was determined by enzyme-linked immunosorbent assay. We recorded the flash electroretinogram (fERG) in dark adapted rats 1 week after LE. At the end of the recording session, the retinas were removed and labeled so that the number of photoreceptors nuclear rows and thickness of the outer nuclear layer was analyzed. RESULTS: Intravitreal injection of BDNF before LE prevented fERG impairment. Different ophthalmic preparations were used for topical eye application; the TSP resulted the most suitable vehicle to increase BDNF level in the retina and optic nerve. Topical eye application with BDNF/TSP before LE partially preserved both fERG response and photoreceptors. CONCLUSIONS: Topical eye treatment with BDNF represents a suitable, noninvasive tool to increase the retinal content of BDNF up to a level capable of exerting neuroprotection toward photoreceptors injured by prolonged LE. TRANSLATIONAL RELEVANCE: A collyrium containing BDNF may serve as an effective, clinically translational treatment against retinal degeneration.
PURPOSE: To test whether the topical eye treatment with BDNF prevents the effects of continuous light exposure (LE) in the albino rat retina. METHODS: Two groups of albino rats were used. The first group of rats received an intraocular injection of BDNF (2 μL, 1 μg/μL) before LE, while the second group was treated with one single drop of BDNF (10 μL, 12 μg/μL) dissolved in different types of solutions (physiological solution, the polysaccharide fraction of Tamarind gum, TSP, and sodium carboxy methyl cellulose), at the level of conjunctival fornix before LE. The level of BDNF in the retina and optic nerve was determined by enzyme-linked immunosorbent assay. We recorded the flash electroretinogram (fERG) in dark adapted rats 1 week after LE. At the end of the recording session, the retinas were removed and labeled so that the number of photoreceptors nuclear rows and thickness of the outer nuclear layer was analyzed. RESULTS: Intravitreal injection of BDNF before LE prevented fERG impairment. Different ophthalmic preparations were used for topical eye application; the TSP resulted the most suitable vehicle to increase BDNF level in the retina and optic nerve. Topical eye application with BDNF/TSP before LE partially preserved both fERG response and photoreceptors. CONCLUSIONS: Topical eye treatment with BDNF represents a suitable, noninvasive tool to increase the retinal content of BDNF up to a level capable of exerting neuroprotection toward photoreceptors injured by prolonged LE. TRANSLATIONAL RELEVANCE: A collyrium containing BDNF may serve as an effective, clinically translational treatment against retinal degeneration.
Authors: Arturo Ortín-Martínez; Francisco Javier Valiente-Soriano; Diego García-Ayuso; Luis Alarcón-Martínez; Manuel Jiménez-López; José Manuel Bernal-Garro; Leticia Nieto-López; Francisco Manuel Nadal-Nicolás; María Paz Villegas-Pérez; Larry A Wheeler; Manuel Vidal-Sanz Journal: PLoS One Date: 2014-12-02 Impact factor: 3.240
Authors: Anthony M Musolf; Claire L Simpson; Bilal A Moiz; Kyle A Long; Laura Portas; Federico Murgia; Elise B Ciner; Dwight Stambolian; Joan E Bailey-Wilson Journal: Invest Ophthalmol Vis Sci Date: 2017-07-01 Impact factor: 4.799