A Di Polo1, L Cheng, G M Bray, A J Aguayo. 1. Center for Research in Neuroscience, Montréal General Hospital Research Institute and McGill University, Montréal, Quebec, Canada. dipoloa@patho.umontreal.ca
Abstract
PURPOSE: To examine the distribution of neurotrophins (NTs) and their catalytic receptors in adult rat photoreceptors. METHODS: Immunocytochemistry and Western blot analyses were performed using primary antibodies raised against NTs (nerve growth factor [NGF], brain-derived neurotrophic factor [BDNF], NT-3, and NT-4/5) and NT receptors (TrkA, TrkB, TrkC, and p75NTR). Double-labeling of retinal sections with opsin-specific antibodies was performed to identify each photoreceptor type. Competitive experiments using excess recombinant NT or Trk receptors confirmed the binding specificity of each antibody. RESULTS: TrkB and BDNF immunoreactivity was colocalized in cone outer segments. TrkB and BDNF were detected in all green-red-sensitive cones, but not in blue-UV cones or rods, and other NTs and NT receptors were not detected in any of the photoreceptor types. CONCLUSIONS: The findings suggest a specific role for BDNF through its signaling receptor TrkB in the function and maintenance of green-red cones, the predominant cone type in the rat retina.
PURPOSE: To examine the distribution of neurotrophins (NTs) and their catalytic receptors in adult rat photoreceptors. METHODS: Immunocytochemistry and Western blot analyses were performed using primary antibodies raised against NTs (nerve growth factor [NGF], brain-derived neurotrophic factor [BDNF], NT-3, and NT-4/5) and NT receptors (TrkA, TrkB, TrkC, and p75NTR). Double-labeling of retinal sections with opsin-specific antibodies was performed to identify each photoreceptor type. Competitive experiments using excess recombinant NT or Trk receptors confirmed the binding specificity of each antibody. RESULTS:TrkB and BDNF immunoreactivity was colocalized in cone outer segments. TrkB and BDNF were detected in all green-red-sensitive cones, but not in blue-UV cones or rods, and other NTs and NT receptors were not detected in any of the photoreceptor types. CONCLUSIONS: The findings suggest a specific role for BDNF through its signaling receptor TrkB in the function and maintenance of green-red cones, the predominant cone type in the rat retina.
Authors: A Germanà; C Sánchez-Ramos; M C Guerrera; M G Calavia; M Navarro; R Zichichi; O García-Suárez; P Pérez-Piñera; Jose A Vega Journal: J Anat Date: 2010-07-21 Impact factor: 2.610
Authors: Ruslan N Grishanin; Haidong Yang; Xiaorong Liu; Kate Donohue-Rolfe; George C Nune; Keling Zang; Baoji Xu; Jacque L Duncan; Matthew M Lavail; David R Copenhagen; Louis F Reichardt Journal: Mol Cell Neurosci Date: 2008-04-22 Impact factor: 4.314
Authors: Eric C Lawson; Moon K Han; Jana T Sellers; Micah A Chrenek; Adam Hanif; Marissa A Gogniat; Jeffrey H Boatright; Machelle T Pardue Journal: J Neurosci Date: 2014-02-12 Impact factor: 6.167
Authors: Baerbel Rohrer; Roman Blanco; Robert E Marc; Marcia B Lloyd; Dean Bok; David M Schneeweis; Louis F Reichardt Journal: Vis Neurosci Date: 2004 Sep-Oct Impact factor: 3.241
Authors: Adam M Hanif; Eric C Lawson; Megan Prunty; Marissa Gogniat; Moe H Aung; Ranjay Chakraborty; Jeffrey H Boatright; Machelle T Pardue Journal: Invest Ophthalmol Vis Sci Date: 2015-10 Impact factor: 4.799