Benjamin D Anderson1, Mengmeng Ma2, Yao Xia2, Tao Wang3, Bo Shu3, John A Lednicky4, Mai-Juan Ma5, Jiahai Lu6, Gregory C Gray7. 1. Department of Environmental & Global Health, College of Public Health & Health Professions, University of Florida, Gainesville Division of Infectious Diseases, School of Medicine and Global Health Institute, Duke University, Durham, North Carolina Department of Medical Statistics and Epidemiology, One Health Research Center, School of Public Health, Sun Yat-sen University, Guangzhou. 2. Department of Medical Statistics and Epidemiology, One Health Research Center, School of Public Health, Sun Yat-sen University, Guangzhou. 3. Zhongshan Center for Disease Control and Prevention Zhongshan Institute, School of Public Health, Sun Yat-sen University, Guangdong Province. 4. Department of Environmental & Global Health, College of Public Health & Health Professions, University of Florida, Gainesville. 5. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology. 6. Department of Medical Statistics and Epidemiology, One Health Research Center, School of Public Health, Sun Yat-sen University, Guangzhou Zhongshan Center for Disease Control and Prevention Zhongshan Institute, School of Public Health, Sun Yat-sen University, Guangdong Province Key Laboratory for Tropical Disease Control, Sun Yat-sen University, Ministry of Education, Guangzhou, Guangdong Province, China. 7. Division of Infectious Diseases, School of Medicine and Global Health Institute, Duke University, Durham, North Carolina.
Abstract
BACKGROUND: Modern agricultural practices create environmental conditions conducive to the emergence of novel pathogens. Current surveillance efforts to assess the burden of emerging pathogens in animal production facilities in China are sparse. In Guangdong Province pig farms, we compared bioaerosol surveillance for influenza A virus to surveillance in oral pig secretions and environmental swab specimens. METHODS: During the 2014 summer and fall/winter seasons, we used 3 sampling techniques to study 5 swine farms weekly for influenza A virus. Samples were molecularly tested for influenza A virus, and positive specimens were further characterized with culture. Risk factors for influenza A virus positivity for each sample type were assessed. RESULTS: Seventy-one of 354 samples (20.1%) were positive for influenza A virus RNA by real-time reverse-transcription polymerase chain reaction analysis. Influenza A virus positivity in bioaerosol samples was a statistically significant predictor for influenza A virus positivity in pig oral secretion and environmental swab samples. Temperature of <20°C was a significant predictor of influenza A virus positivity in bioaerosol samples. DISCUSSIONS: Climatic factors and routine animal husbandry practices may increase the risk of human exposure to aerosolized influenza A viruses in swine farms. Data suggest that bioaerosol sampling in pig barns may be a noninvasive and efficient means to conduct surveillance for novel influenza viruses.
BACKGROUND: Modern agricultural practices create environmental conditions conducive to the emergence of novel pathogens. Current surveillance efforts to assess the burden of emerging pathogens in animal production facilities in China are sparse. In Guangdong Province pig farms, we compared bioaerosol surveillance for influenza A virus to surveillance in oral pig secretions and environmental swab specimens. METHODS: During the 2014 summer and fall/winter seasons, we used 3 sampling techniques to study 5 swine farms weekly for influenza A virus. Samples were molecularly tested for influenza A virus, and positive specimens were further characterized with culture. Risk factors for influenza A virus positivity for each sample type were assessed. RESULTS: Seventy-one of 354 samples (20.1%) were positive for influenza A virus RNA by real-time reverse-transcription polymerase chain reaction analysis. Influenza A virus positivity in bioaerosol samples was a statistically significant predictor for influenza A virus positivity in pig oral secretion and environmental swab samples. Temperature of <20°C was a significant predictor of influenza A virus positivity in bioaerosol samples. DISCUSSIONS: Climatic factors and routine animal husbandry practices may increase the risk of human exposure to aerosolized influenza A viruses in swine farms. Data suggest that bioaerosol sampling in pig barns may be a noninvasive and efficient means to conduct surveillance for novel influenza viruses.
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