Literature DB >> 27187075

When Wavelengths Collide: Bias in Cell Abundance Measurements Due to Expressed Fluorescent Proteins.

Ariel Hecht1,2,3, Drew Endy1,3, Marc Salit1,2,3, Matthew S Munson1,2,3.   

Abstract

The abundance of bacteria in liquid culture is commonly inferred by measuring optical density at 600 nm. Red fluorescent proteins (RFPs) can strongly absorb light at 600 nm. Increasing RFP expression can falsely inflate apparent cell density and lead to underestimations of mean per-cell fluorescence by up to 10%. Measuring optical density at 700 nm would allow estimation of cell abundance unaffected by the presence of nearly all fluorescent proteins.

Entities:  

Keywords:  OD600; OD700; RFP; abundance; optical density

Mesh：

Substances：

Year:  2016        PMID： 27187075     DOI： 10.1021/acssynbio.6b00072

Source DB:  PubMed          Journal:  ACS Synth Biol        ISSN： 2161-5063            Impact factor:   5.110


  10 in total

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7.  Development of High-Performance Whole Cell Biosensors Aided by Statistical Modeling.

Authors:  Adokiye Berepiki; Ross Kent; Leopoldo F M Machado; Neil Dixon
Journal:  ACS Synth Biol       Date:  2020-02-17       Impact factor: 5.110

8.  Robust estimation of bacterial cell count from optical density.

Authors:  Jacob Beal; Natalie G Farny; Traci Haddock-Angelli; Vinoo Selvarajah; Geoff S Baldwin; Russell Buckley-Taylor; Markus Gershater; Daisuke Kiga; John Marken; Vishal Sanchania; Abigail Sison; Christopher T Workman
Journal:  Commun Biol       Date:  2020-09-17

9.  Droplet printing reveals the importance of micron-scale structure for bacterial ecology.

Authors:  Ravinash Krishna Kumar; Thomas A Meiller-Legrand; Alessandro Alcinesio; Diego Gonzalez; Despoina A I Mavridou; Oliver J Meacock; William P J Smith; Linna Zhou; Wook Kim; Gökçe Su Pulcu; Hagan Bayley; Kevin R Foster
Journal:  Nat Commun       Date:  2021-02-08       Impact factor: 14.919

10.  Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation.

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  10 in total

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