| Literature DB >> 27179568 |
Tomáš Riedel1, František Surman2, Simone Hageneder3, Ognen Pop-Georgievski2, Christa Noehammer4, Manuela Hofner4, Eduard Brynda2, Cesar Rodriguez-Emmenegger5, Jakub Dostálek6.
Abstract
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.Entities:
Keywords: Antifouling; Biosensors; Blood serum; Hepatitis B; Polymer brushes; Surface plasmon resonance
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Year: 2016 PMID： 27179568 DOI： 10.1016/j.bios.2016.05.014
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618