Literature DB >> 2717404

Purification and characterization of two serine isoacceptor tRNAs from bovine mitochondria by using a hybridization assay method.

T Yokogawa1, Y Kumazawa, K Miura, K Watanabe.   

Abstract

For large scale preparation of mitochondrial tRNAs, a new hybridization assay method using synthetic oligodeoxyribonucleotide probes (16-17mer) complementary to individual tRNA sequences was developed and applied for the purification of two serine isoacceptor tRNAs (tRNASerAGY and tRNASerUCN) from bovine mitochondria. It is about 100 times more sensitive than the conventional aminoacylation assay method. 2-4 A260 units each of both tRNASer isoacceptors were purified from 17.5 kg of bovine liver, and they were characterized by means of nuclease digestion, melting profiles and aminoacylation activity. It is suggested that tRNASerUCN possesses the D loop/T loop interaction like usual L-shaped tRNAs, and that tRNASerAGY lacking almost an entire D arm is aminoacylated with an efficiency not very much lower than that of tRNASerUCN.

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Year:  1989        PMID： 2717404      PMCID： PMC317647          DOI： 10.1093/nar/17.7.2623

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  30 in total

Review 1.  Recent excitement in understanding transfer RNA identity.

Authors:  L H Schulman; J Abelson
Journal:  Science       Date:  1988-06-17       Impact factor: 47.728

2.  Analysis of modified nucleosides and nucleotide sequence of tRNA.

Authors:  Y Kuchino; N Hanyu; S Nishimura
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

3.  Bizarre tRNAs inferred from DNA sequences of mitochondrial genomes of nematode worms.

Authors:  D R Wolstenholme; J L Macfarlane; R Okimoto; D O Clary; J A Wahleithner
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

4.  Ser-tRNAs from bovine mitochondrion form ternary complexes with bacterial elongation factor Tu and GTP.

Authors:  E Gebhardt-Singh; M Sprinzl
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

5.  Unusual genetic codes and a novel gene structure for tRNA(AGYSer) in starfish mitochondrial DNA.

Authors:  H Himeno; H Masaki; T Kawai; T Ohta; I Kumagai; K Miura; K Watanabe
Journal:  Gene       Date:  1987       Impact factor: 3.688

Review 6.  Compilation of tRNA sequences and sequences of tRNA genes.

Authors:  M Sprinzl; T Hartmann; F Meissner; J Moll; T Vorderwülbecke
Journal:  Nucleic Acids Res       Date:  1987       Impact factor: 16.971

7.  Bovine mitochondrial ribosomes. Elongation factor specificity.

Authors:  S L Eberly; V Locklear; L L Spremulli
Journal:  J Biol Chem       Date:  1985-07-25       Impact factor: 5.157

8.  The complete nucleotide sequence of the Xenopus laevis mitochondrial genome.

Authors:  B A Roe; D P Ma; R K Wilson; J F Wong
Journal:  J Biol Chem       Date:  1985-08-15       Impact factor: 5.157

9.  Nucleotide sequence and gene organization of sea urchin mitochondrial DNA.

Authors:  H T Jacobs; D J Elliott; V B Math; A Farquharson
Journal:  J Mol Biol       Date:  1988-07-20       Impact factor: 5.469

10.  Clustering of tRNA genes in Paracentrotus lividus mitochondrial DNA.

Authors:  P Cantatore; M Roberti; G Rainaldi; C Saccone; M N Gadaleta
Journal:  Curr Genet       Date:  1988       Impact factor: 3.886
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  22 in total

1.  Expression of bovine mitochondrial tRNASer GCU derivatives in Escherichia coli.

Authors:  I Hayashi; G Kawai; K Watanabe
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

2.  Nucleotide sequence of tryptophan tRNA from Bacillus subtilis.

Authors:  J Matsugi; K Murao; H Ishikura
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Strand-specific nucleotide composition bias in echinoderm and vertebrate mitochondrial genomes.

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4.  Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization.

Authors:  Y Kumazawa; T Yokogawa; H Tsurui; K Miura; K Watanabe
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

5.  Higher-order structure of bovine mitochondrial tRNA(SerUGA): chemical modification and computer modeling.

Authors:  Y Watanabe; G Kawai; T Yokogawa; N Hayashi; Y Kumazawa; T Ueda; K Nishikawa; I Hirao; K Miura; K Watanabe
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971

6.  Sequence evolution of mitochondrial tRNA genes and deep-branch animal phylogenetics.

Authors:  Y Kumazawa; M Nishida
Journal:  J Mol Evol       Date:  1993-10       Impact factor: 2.395

7.  Comparison of tRNA conformation during different phases of reproduction.

Authors:  M Sarkar; M Vinayak
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8.  A novel cloverleaf structure found in mammalian mitochondrial tRNA(Ser) (UCN).

Authors:  T Yokogawa; Y Watanabe; Y Kumazawa; T Ueda; I Hirao; K Miura; K Watanabe
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

9.  Optimization of the hybridization-based method for purification of thermostable tRNAs in the presence of tetraalkylammonium salts.

Authors:  Takashi Yokogawa; Yusuke Kitamura; Daigo Nakamura; Satoshi Ohno; Kazuya Nishikawa
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10.  Tertiary network in mammalian mitochondrial tRNAAsp revealed by solution probing and phylogeny.

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Journal:  Nucleic Acids Res       Date:  2009-09-18       Impact factor: 16.971
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