| Literature DB >> 27159899 |
Guoliang Ke1,2, Minghui Liu1, Shuoxing Jiang1, Xiaodong Qi1, Yuhe Renee Yang1, Shaun Wootten1, Fei Zhang1, Zhi Zhu2, Yan Liu3, Chaoyong James Yang4, Hao Yan5.
Abstract
Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH-MDH and G6pDH-LDH) through the control of NAD(+) substrate channeling by specifically shifting NAD(+) between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.Entities:
Keywords: DNA origami; NADH; enzymes; self-assembly; substrate channeling
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Year: 2016 PMID： 27159899 DOI： 10.1002/anie.201603183
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336