Sung Hwan Cho1, Ki Wha Chung2, Jung Oh Kim3, Hyogeun Jang3, Jung Ki Yoo3, Youngsok Choi3, Jung Jae Ko3, Ji Hyang Kim4, Yoshihiro Nishi5, Toshihiko Yanase5, Woo Sik Lee6, Nam Keun Kim7. 1. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, 463-712, South Korea. 2. Department of Biological Science, College of Natural Science, Kongju National University, Gongju, South Korea. 3. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea. 4. Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea. 5. Department of Endocrinology and Diabetes, School of Medicine, Fukuoka University, 814-0180, Japan. 6. Fertility Center of CHA Gangnam Medical Center, CHA University, Gangnam, 135-081, South Korea. Electronic address: wooslee@cha.ac.kr. 7. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, 463-712, South Korea. Electronic address: nkkim@cha.ac.kr.
Abstract
OBJECTIVE: The aim of this study was to investigate the association of microRNA polymorphisms (miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G) in Korean patients with recurrent implantation failure (RIF). METHODS: We conducted a case-control study of 354 Korean women: 120 patients with RIF and 234 healthy controls with at least one live birth and no history of pregnancy loss. RESULTS: The combined miR-146aCG+GG/miR-196a2CC genotype was more frequent in patients than in controls (P<0.05), and apparently conferred increased susceptibility. Conversely, genotype-based multifactor dimensionality reduction analysis, revealed that the G-T-T-A (miR-146a/-149/-196a2/-499) and G-T-T inferred genotypes (miR-146a/-149/-196a2) were significantly less frequent in patients, which suggested potential protective effects. The expression of miR-146a for the GG homozygote was significantly lower (P<0.05) than expression of the CC homozygote from both the pre, mature and sequences of miR-146a-3p (P<0.05 each). The expression of miR-196a2 for the CC homozygote was also lower than the TT homozygote from the mature sequence of miR-196a2-3p (P<0.05). CONCLUSIONS: These results suggest that the polymorphisms in miR-146a and miR-196a2 could alter their target mRNA expression. Our findings suggest that expression levels of miR-146aC>G, miR-196a2T>C and putative gene-gene interaction between miR-146a, miR-196a2, miR-149 may be involved in RIF development in Korean women.
OBJECTIVE: The aim of this study was to investigate the association of microRNA polymorphisms (miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G) in Korean patients with recurrent implantation failure (RIF). METHODS: We conducted a case-control study of 354 Korean women: 120 patients with RIF and 234 healthy controls with at least one live birth and no history of pregnancy loss. RESULTS: The combined miR-146aCG+GG/miR-196a2CC genotype was more frequent in patients than in controls (P<0.05), and apparently conferred increased susceptibility. Conversely, genotype-based multifactor dimensionality reduction analysis, revealed that the G-T-T-A (miR-146a/-149/-196a2/-499) and G-T-T inferred genotypes (miR-146a/-149/-196a2) were significantly less frequent in patients, which suggested potential protective effects. The expression of miR-146a for the GG homozygote was significantly lower (P<0.05) than expression of the CC homozygote from both the pre, mature and sequences of miR-146a-3p (P<0.05 each). The expression of miR-196a2 for the CC homozygote was also lower than the TT homozygote from the mature sequence of miR-196a2-3p (P<0.05). CONCLUSIONS: These results suggest that the polymorphisms in miR-146a and miR-196a2 could alter their target mRNA expression. Our findings suggest that expression levels of miR-146aC>G, miR-196a2T>C and putative gene-gene interaction between miR-146a, miR-196a2, miR-149 may be involved in RIF development in Korean women.
Authors: Lívia Reis Silva; Anderson Sanches Melo; Cristiana Libardi Miranda Furtado; Rui Alberto Ferriani; Karina Bezerra Salomão; Suleimy Cristina Mazin; Luiz Gonzaga Tone; Viviane Cunha Cardoso; Rosana Maria Dos Reis Journal: J Assist Reprod Genet Date: 2022-06-11 Impact factor: 3.357