Ge Shi1, Tingting Wang1, Shijie Li1, Yunlong Cheng1, Ping Sheng1, Yiming Fan1, KunJu Zhu2. 1. Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China. 2. Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China; First Affiliated Hospital and Institute of Mycology, Jinan University, Guangzhou, Guangdong, China. Electronic address: zhukunju1111@163.com.
Abstract
BACKGROUND: The toll-like receptors-(TLR)-2 and -4 play a role in the innate immune system and drive the autoimmune T cell cascade in psoriasis. But little is known about the association of the polymorphisms of TLR2/TLR4 and psoriasis vulgaris (PsV). METHODS: We performed a hospital-based association study involved in355 PsV patients and 213 controls. Genotyping of 11 SNPs rs4696480(-16934A/T), rs121917864 (2029C/T;Arg677Trp), rs5743708 (2408G/A;Arg753Gln), rs3804099 (+597T/C), rs5743699(+1349T/C) of TLR2 on chromosome 4q32; and rs1927914 (-6143A/G), rs10759932(-5724T/C), rs4986790 (896A/G; Asp299Gly), rs4986791 (1196C/T; Thr399lle); rs11536889(+7263G/C), rs11536891(+8469T/C) of TLR4 on chromosome 9q33.1 were determined using SNaPshot Multiplex Kit (Applied Biosystems Co., USA). RESULTS: We did not detect the presence of any of the five rare SNPs (rs5743708, rs121917864, rs5743699, rs4986790, rs4986791). The comparison of allele distributions revealed that only one SNP (rs3804099) of 6 common SNPs(rs10759932, rs11536889, rs11536891, rs1927914, rs3804099, rs4696480) was significant associated with the risk of PsV (P<0.01; FDR p-Value). However, there were no significant differences found in other subphenotypes of family history or PASI between patients positive and those negative for a particular phenotype. CONCLUSIONS: The SNP rs3804099 of TLR2 may have significant effects on heritability of PsV in our Chinese population. TLR2 and its pathway might take part in the pathogenesis of PsV.
BACKGROUND: The toll-like receptors-(TLR)-2 and -4 play a role in the innate immune system and drive the autoimmune T cell cascade in psoriasis. But little is known about the association of the polymorphisms of TLR2/TLR4 and psoriasis vulgaris (PsV). METHODS: We performed a hospital-based association study involved in355 PsV patients and 213 controls. Genotyping of 11 SNPs rs4696480(-16934A/T), rs121917864 (2029C/T;Arg677Trp), rs5743708 (2408G/A;Arg753Gln), rs3804099 (+597T/C), rs5743699(+1349T/C) of TLR2 on chromosome 4q32; and rs1927914 (-6143A/G), rs10759932(-5724T/C), rs4986790 (896A/G; Asp299Gly), rs4986791 (1196C/T; Thr399lle); rs11536889(+7263G/C), rs11536891(+8469T/C) of TLR4 on chromosome 9q33.1 were determined using SNaPshot Multiplex Kit (Applied Biosystems Co., USA). RESULTS: We did not detect the presence of any of the five rare SNPs (rs5743708, rs121917864, rs5743699, rs4986790, rs4986791). The comparison of allele distributions revealed that only one SNP (rs3804099) of 6 common SNPs(rs10759932, rs11536889, rs11536891, rs1927914, rs3804099, rs4696480) was significant associated with the risk of PsV (P<0.01; FDR p-Value). However, there were no significant differences found in other subphenotypes of family history or PASI between patients positive and those negative for a particular phenotype. CONCLUSIONS: The SNP rs3804099 of TLR2 may have significant effects on heritability of PsV in our Chinese population. TLR2 and its pathway might take part in the pathogenesis of PsV.