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Literature DB >> 27147036

Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).

Shimi Takeshi^1,2, Chan-Gi Pack³, Robert D Goldman⁴.

Abstract

The major structural components of the nuclear lamina are the A- and B-type nuclear lamin proteins which are also present in the nucleoplasm. Studies of molecular movements of the lamins in both the lamina and nucleoplasm of living cell nuclei have provided insights into their roles in maintaining nuclear architecture. In this chapter, we present protocols for quantitatively measuring the mobilities of lamin proteins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) in mammalian cell nuclei.

Entities: CellLine Chemical Disease Gene Mutation Species

Keywords: FCS; FRAP; Lamin; Lamina

Mesh：

Substances：

Year: 2016 PMID： 27147036 PMCID： PMC5472422 DOI： 10.1007/978-1-4939-3530-7_5

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

12 in total

1. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.

Authors: Lothar Schermelleh; Peter M Carlton; Sebastian Haase; Lin Shao; Lukman Winoto; Peter Kner; Brian Burke; M Cristina Cardoso; David A Agard; Mats G L Gustafsson; Heinrich Leonhardt; John W Sedat
Journal: Science Date: 2008-06-06 Impact factor: 47.728

Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).

1. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.

2. The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription.

3. Distinct structural and mechanical properties of the nuclear lamina in Hutchinson-Gilford progeria syndrome.

4. Structural studies on lamin. Similarities and differences between lamin and intermediate-filament proteins.

Review 5. Insights into the differences between the A- and B-type nuclear lamins.

6. The nuclear lamina is a meshwork of intermediate-type filaments.

7. Reversal of the cellular phenotype in the premature aging disease Hutchinson-Gilford progeria syndrome.

8. Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells.

9. Interphase phosphorylation of lamin A.

10. Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins.

1. Promoter hypermethylation as a mechanism for Lamin A/C silencing in a subset of neuroblastoma cells.

Review 2. A Perspective on the Experimental Techniques for Studying Lamins.